ACSM5 Polyclonal antibody

ACSM5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16591-1-AP

Synonyms

MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5

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Validation Data Gallery

• 

  WB analysis of mouse liver using 16591-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16591-1-AP (ACSM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16591-1-AP targets ACSM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSM5 fusion protein Ag9373
• Full Name: acyl-CoA synthetase medium-chain family member 5
• Calculated Molecular Weight: 208 aa, 23 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC016703
• Gene Symbol: ACSM5
• Gene ID (NCBI): 54988
• RRID: AB_2878284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Mol Metab; Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis; Authors - Yuan Gui