Tested Applications
| Positive WB detected in | HepG2 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16204-1-AP targets ACSS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9173 Product name: Recombinant human ACSS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 341-686 aa of BC009317 Sequence: DLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNVMILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKITSTIEDPSIFGHVEEMLKQA Predict reactive species |
| Full Name | acyl-CoA synthetase short-chain family member 3 |
| Calculated Molecular Weight | 686 aa, 75 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC009317 |
| Gene Symbol | ACSS3 |
| Gene ID (NCBI) | 79611 |
| RRID | AB_2257893 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6R3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACSS3(acyl-CoA synthetase short-chain family member 3, mitochondrial) belongs to the ATP-dependent AMP-binding enzyme family. It activates acetate so that it can be used for lipid synthesis or for energy generation. The deduced 686-amino acid protein contains 4 of 5 motifs characteristic of acyl-CoA synthetases. This protein has 2 isoforms produced by alternative splicing. The full length protein has a transit peptide with 29 amino acids.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACSS3 antibody 16204-1-AP | Download protocol |
| IHC protocol for ACSS3 antibody 16204-1-AP | Download protocol |
| IP protocol for ACSS3 antibody 16204-1-AP | Download protocol |
| WB protocol for ACSS3 antibody 16204-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACSS2 antibody (Cat# 16204-1-AP) has 13 total citations. Citations appear in journals including Cell Metabolism, Nature Communications, Cell Reports, Cancer Communications, Cancer Genetics, Communications Biology, Nutrients, BBA Molecular Basis of Disease, Acta Biochimica et Biophysica Sinica, Biochemical Journal, Food and Chemical Toxicology, PLoS One, and Translational Cancer Research. Research fields span liver disease (NAFLD, hepatotoxicity), oncology (glioblastoma, ovarian, neuroblastoma, esophageal, lung cancers), metabolic regulation, ferroptosis, pulmonary fibrosis, and diabetes.
| Species | Application | Title |
|---|---|---|
Cell Metab Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11 | ||
Cancer Commun (Lond) Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma | ||
Nat Commun ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expression | ||
Cancer Genet Prognosis prediction and drug guidance of ovarian serous cystadenocarcinoma through mitochondria gene-based model | ||
Cell Rep High fructose promotes MYCN-amplified neuroblastoma progression through NgBR-ACSS2-mediated biosynthesis of acetyl-CoA | ||
Commun Biol BCL11A deficiency protects epidermis from UVB-induced damage through promotion of autophagy. |
