ACTL7A Polyclonal antibody

ACTL7A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17355-1-AP

Synonyms

Actin like 7 alpha, actin like 7A, Actin like protein 7A, ACTL7A

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Validation Data Gallery

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  WB analysis using 17355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17355-1-AP (ACTL7A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 17355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17355-1-AP

  Immunofluorescent analysis of HepG2 cells, using ACTL7A antibody 17355-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Product Information

17355-1-AP targets ACTL7A in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACTL7A fusion protein Ag10613
• Full Name: actin-like 7A
• Calculated molecular weight: 435 aa, 49 kDa
• Observed molecular weight: 49 kDa
• GenBank accession number: BC014610
• Gene symbol: ACTL7A
• Gene ID (NCBI): 10881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.

Publications

Species	Application	Title
human	IF,CoIP	Am J Hum Genet; Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice.; Authors - Jing Dai
human	CoIP	Hum Reprod; Loss of SPACA1 function causes autosomal recessive globozoospermia by damaging the acrosome-acroplaxome complex.; Authors - Pingping Chen
human	WB,IF	Hum Reprod; Novel bi-allelic variants in ACTL7A are associated with male infertility and total fertilization failure.; Authors - Jing Wang
human,mouse	WB,IF,IP	Development; Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.; Authors - Xiao-Zhen Zhang
human,mouse	WB,IF,IP	Mol Hum Reprod; Pathogenic variant in ACTL7A causes severe teratozoospermia characterized by bubble-shaped acrosomes and male infertility.; Authors - Jing Dai
human,mouse	IF,CoIP	Cell Rep; The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice.; Authors - Xiao-Zhen Zhang