ACTL7A Polyclonal antibody

ACTL7A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17355-1-AP


Actin like 7 alpha, actin like 7A, Actin like protein 7A, ACTL7A

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17355-1-AP targets ACTL7A in WB, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTL7A fusion protein Ag10613
Full Name actin-like 7A
Calculated molecular weight 435 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC014610
Gene symbol ACTL7A
Gene ID (NCBI) 10881
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.


Product Specific Protocols
WB protocol for ACTL7A antibody 17355-1-APDownload protocol
IHC protocol for ACTL7A antibody 17355-1-APDownload protocol
IF protocol for ACTL7A antibody 17355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Hum Reprod

Loss of SPACA1 function causes autosomal recessive globozoospermia by damaging the acrosome-acroplaxome complex.

Authors - Pingping Chen

Hum Reprod

Novel bi-allelic variants in ACTL7A are associated with male infertility and total fertilization failure.

Authors - Jing Wang

Am J Hum Genet

Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice.

Authors - Jing Dai