ACTL7A Polyclonal antibody

ACTL7A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17355-1-AP

Synonyms

Actin like 7 alpha, actin like 7A, Actin like protein 7A, ACTL7A

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Validation Data Gallery

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  WB analysis using 17355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17355-1-AP (ACTL7A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 17355-1-AP

  Immunofluorescent analysis of HepG2 cells, using ACTL7A antibody 17355-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IHC staining of mouse testis using 17355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Product Information

17355-1-AP targets ACTL7A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACTL7A fusion protein Ag10613
• Full Name: actin-like 7A
• Calculated molecular weight: 435 aa, 49 kDa
• Observed molecular weight: 49 kDa
• GenBank accession number: BC014610
• Gene symbol: ACTL7A
• Gene ID (NCBI): 10881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.