ACTL7A Polyclonal antibody
ACTL7A Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17355-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
17355-1-AP targets ACTL7A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACTL7A fusion protein Ag10613 |
Full Name | actin-like 7A |
Calculated molecular weight | 435 aa, 49 kDa |
Observed molecular weight | 49 kDa |
GenBank accession number | BC014610 |
Gene symbol | ACTL7A |
Gene ID (NCBI) | 10881 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.
Protocols
Product Specific Protocols | |
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WB protocol for ACTL7A antibody 17355-1-AP | Download protocol |
IHC protocol for ACTL7A antibody 17355-1-AP | Download protocol |
IF protocol for ACTL7A antibody 17355-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |