|Positive WB detected in||mouse testis tissue, rat testis tissue|
|Positive IHC detected in||mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17355-1-AP targets ACTL7A in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACTL7A fusion protein Ag10613|
|Full Name||actin-like 7A|
|Calculated molecular weight||435 aa, 49 kDa|
|Observed molecular weight||49 kDa|
|GenBank accession number||BC014610|
|Gene ID (NCBI)||10881|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.
Am J Hum Genet
Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice.
ACROSIN deficiency causes total fertilization failure in humans by preventing the sperm from penetrating the zona pellucida
Loss of SPACA1 function causes autosomal recessive globozoospermia by damaging the acrosome-acroplaxome complex.
Novel bi-allelic variants in ACTL7A are associated with male infertility and total fertilization failure.
Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.
Mol Hum Reprod
Pathogenic variant in ACTL7A causes severe teratozoospermia characterized by bubble-shaped acrosomes and male infertility.