ACTN2 Polyclonal antibody

ACTN2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14221-1-AP


actinin, alpha 2, ACTN2, Alpha actinin 2, CMD1AA, F actin cross linking protein

Tested Applications

Positive WB detected inmouse brain tissue, C2C12 cells, mouse lung tissue, mouse skeletal muscle tissue, mouse heart, mouse kidney, rat skeletal muscle
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHuman iPSC derived cardiomyocyte, NIH/3T3 cells, C2C12 cells, mouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14221-1-AP targets ACTN2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN2 fusion protein Ag5459
Full Name actinin, alpha 2
Calculated molecular weight 104 kDa
Observed molecular weight 103 kDa
GenBank accession numberBC051770
Gene symbol ACTN2
Gene ID (NCBI) 88
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Product Specific Protocols
WB protocol for ACTN2 antibody 14221-1-APDownload protocol
IHC protocol for ACTN2 antibody 14221-1-APDownload protocol
IF protocol for ACTN2 antibody 14221-1-APDownload protocol
IP protocol for ACTN2 antibody 14221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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