ACTN2 Polyclonal antibody

ACTN2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14221-1-AP

Synonyms

actinin, alpha 2, ACTN2, Alpha actinin 2, CMD1AA, F actin cross linking protein



Tested Applications

Positive WB detected inrat skeletal muscle tissue, C2C12 cells, mouse lung tissue, mouse skeletal muscle tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, C2C12 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

14221-1-AP targets ACTN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN2 fusion protein Ag5459
Full Name actinin, alpha 2
Calculated molecular weight 104 kDa
Observed molecular weight 103 kDa
GenBank accession numberBC051770
Gene symbol ACTN2
Gene ID (NCBI) 88
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACTN2 antibody 14221-1-APDownload protocol
IHC protocol for ACTN2 antibody 14221-1-APDownload protocol
IF protocol for ACTN2 antibody 14221-1-APDownload protocol
IP protocol for ACTN2 antibody 14221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Cell Biochem

Possible mechanism of GATA4 inhibiting myocardin activity during cardiac hypertrophy.

Authors - Yao Xu
mouseWB

Biochem Biophys Res Commun

MiR-466h-5p induces expression of myocardin with complementary promoter sequences.

Authors - Ying Luo
ratWB

Dokl Biochem Biophys

Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Authors - I O Petrova
humanWB

Prog Biophys Mol Biol

Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro.

Authors - Xueling He