ACTN2 Polyclonal antibody
ACTN2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 14221-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, C2C12 cells, mouse lung tissue, mouse skeletal muscle tissue, mouse heart, mouse kidney, rat skeletal muscle|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse heart tissue, mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Human iPSC derived cardiomyocyte, mouse heart tissue, C2C12 cells, NIH/3T3 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
|WB||See 10 publications below|
|IHC||See 2 publications below|
|IF||See 8 publications below|
14221-1-AP targets ACTN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACTN2 fusion protein Ag5459|
|Full Name||actinin, alpha 2|
|Calculated molecular weight||104 kDa|
|Observed molecular weight||103 kDa|
|GenBank accession number||BC051770|
|Gene ID (NCBI)||88|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).
|Product Specific Protocols|
|WB protocol for ACTN2 antibody 14221-1-AP||Download protocol|
|IHC protocol for ACTN2 antibody 14221-1-AP||Download protocol|
|IF protocol for ACTN2 antibody 14221-1-AP||Download protocol|
|IP protocol for ACTN2 antibody 14221-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.
Basic Res Cardiol
Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.
Essential role of MESP1-RING1A complex in cardiac differentiation
Front Cell Dev Biol
Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy.
Front Cell Dev Biol
Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
J Mol Cell Cardiol
LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Rebecca (Verified Customer) (09-21-2022)
20ug of protein was loaded. Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC.