ACTR1B Antibody

ACTR1B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11023-1-AP

Synonyms

Actin related protein 1B, ACTR1B, ARP1B, Beta centractin, CTRN2, PC3



Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:300
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11023-1-AP targets ACTR1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTR1B fusion protein Ag1467
Full Name ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Calculated molecular weight 42 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC010090
Gene symbol ACTR1B
Gene ID (NCBI) 10120
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTR1B (also known as beta centractin) is a part of the 20S dynactin complex. It colocalizes with centrosomes and vesicular (membranous) structures and is involved in cell division. ACTR1B and ACTR1A share 90% amino acid identity, and this antibody recognizes both of ACTR1B and ACTR1A.

Protocols

Product Specific Protocols
WB protocol for ACTR1B antibody 11023-1-APDownload protocol
IHC protocol for ACTR1B antibody 11023-1-APDownload protocol
IF protocol for ACTR1B antibody 11023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols