ACY1 Recombinant antibody

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  WB analysis using 86204-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86204-1-RR (ACY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ACY1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86204-1-RR

Clone No.250846D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

aminoacylase 1, ACY 1, ACY-1, Aminoacylase-1, EC:3.5.1.14

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, PC-3 cells, Neuro-2a cells, NIH/3T3 cells, PC-12 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86204-1-RR targets ACY1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACY1 fusion protein Ag1813
• Full Name: aminoacylase 1
• Calculated Molecular Weight: 40.8 kDa
• GenBank Accession Number: BC014112
• Gene Symbol: ACY1
• Gene ID (NCBI): 95
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q03154
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aminoacylase 1 (ACY1) is a cytosolic enzyme that is widely distributed in mammalian tissues and catalyzes the hydrolysis of acylated amino acids into amino acids and acyl groups. The main functions of ACY1 are to accelerate the hydrolysis of N-acetylated peptides, especially N-acetylated neutral aliphatic amino acids, and participate in protein synthesis and turnover through the release of free amino acids.