ADAM12 Polyclonal antibody

• Featured Product
• KD/KO Validated

ADAM12 Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 14139-1-AP

Synonyms

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 14139-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 14139-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14139-1-AP

  IP Result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug.

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14139-1-AP

  Immunofluorescent analysis of HepG2 cells, using ADAM12 antibody 14139-1-AP at 1:25 and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 20 publications below
• IHC: See 13 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Product Information

14139-1-AP targets ADAM12 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM12 fusion protein Ag5206
• Full Name: ADAM metallopeptidase domain 12
• Calculated molecular weight: 100 kDa
• Observed molecular weight: 90-100 kDa, 70-80 kDa
• GenBank accession number: BC060804
• Gene symbol: ADAM12
• Gene ID (NCBI): 8038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.

Publications

Species	Application	Title
human	WB,IHC	Int J Mol Med; ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.; Authors - Wei Li
human	WB	Nucleic Acids Res; Genetic screens reveal mechanisms for the transcriptional regulation of tissue-specific genes in normal cells and tumors.; Authors - Ikrame Naciri
mouse	IHC	Calcif Tissue Int; Involvement of ADAM12 in Chondrocyte Differentiation by Regulation of TGF-β1-Induced IGF-1 and RUNX-2 Expressions.; Authors - Masahiro Horita ; KO Validated
human	WB	J Proteome Res; SILAC Quantitative Proteomics and Biochemical Analyses Reveal a Novel Molecular Mechanism by which ADAM12S Promotes the Proliferation, Migration, and Invasion of Small Cell Lung Cancer Cells through Upregulating Hexokinase 1.; Authors - Qianqian Duan ; KD Validated
mouse	FC	Cell Rep; Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations.; Authors - Abigail P Leinroth
human	WB	Pathol Res Pract; Methylation status of ADAM12 promoter are associated with its expression levels in colorectal cancer.; Authors - Yan Wang

Related Products

SH3PXD2A Polyclonal antibody

18976-1-AP

IGFBP1 Polyclonal antibody

13981-1-AP

SRC Polyclonal antibody

11097-1-AP

TGF Beta 1 Polyclonal antibody

18978-1-AP

RACK1 Polyclonal antibody

10076-1-AP

SKIL Polyclonal antibody

19218-1-AP