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ADAM12 Antibody 25 Publications

Rabbit Polyclonal| Catalog number: 14139-1-AP

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Con: 39 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

ADAM12 Antibody WB mouse brain tissue 14139-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ADAM12 Antibody IP HeLa cells 14139-1-AP

IP Result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug.

ADAM12 Antibody IHC human breast cancer tissue 14139-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP(ADAM12 antibody) at dilution of 1:50 (under 10x lens)

ADAM12 Antibody IHC human breast cancer tissue 14139-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP(ADAM12 antibody) at dilution of 1:50 (under 40x lens)

ADAM12 Antibody IF HepG2 cells 14139-1-AP

Immunofluorescent analysis of HepG2 cells, using ADAM12 antibody 14139-1-AP at 1:25 and Rhodamine-labeled goat anti-rabbit IgG (red).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, Y79 cells, mouse heart tissue, mouse colon tissue, human placenta tissue, HeLa cells, MCF-7 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue, human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ADAM12 fusion protein Ag5206
Full name:
ADAM metallopeptidase domain 12
Calculated molecular weight:
100 kDa
Observed molecular weight:
90-100 kDa, 70-80 kDa
GenBank accession number:
BC060804
Gene ID (NCBI):
8038
Gene symbol
ADAM12
Synonyms
ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA

Background

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors. It is involved in the regulation of growth factor activities and integrin functions, leading to promotion of cell growth and invasion, although the precise mechanisms of these are not clear at the present time.(PMID:17355265) ADAM12’s ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein’s role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kd full-length form, 90kd mature (processed form that lack the prodomain); 50-68kd degradation product and 27kd prodomain.


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