ADAM12 Polyclonal antibody

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  WB analysis of mouse brain using 14139-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14139-1-AP

  IP result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug.

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14139-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ADAM12 antibody (14139-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 14139-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADAM12 antibody (14139-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14139-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ADAM12 Polyclonal antibody (14139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

ADAM12 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14139-1-AP

Publications(43)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

MLTN, Meltrin-alpha, MCMP, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 12

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, mouse heart tissue, rat brain tissue, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 26 publications below
• IHC: See 16 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Product Information

14139-1-AP targets ADAM12 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM12 fusion protein Ag5206
• Full Name: ADAM metallopeptidase domain 12
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 90-100 kDa, 70-80 kDa
• GenBank Accession Number: BC060804
• Gene Symbol: ADAM12
• Gene ID (NCBI): 8038
• RRID: AB_2289230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43184
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.

Publications

Species	Application	Title
mouse,human	IF	Cell Stem Cell; Vaccination-based immunotherapy to target profibrotic cells in liver and lung; Authors - Michal Sobecki
human	IHC	J Cell Biol; Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.; Authors - Díaz Bego?a B
		Matrix Biol; Antibodies and methods for immunohistochemistry of extracellular matrix proteins.; Authors - Steffen Rickelt
human	IF	Theranostics; Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.; Authors - Yinan Chen
human	WB,IF	Cell Death Differ; ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion.; Authors - M Aghababaei
human	IHC	J Hematol Oncol; Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway.; Authors - Kosuke Ogawa