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  • KD/KO Validated

ADAM12 Polyclonal antibody

ADAM12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14139-1-AP

Synonyms

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA



Tested Applications

Positive WB detected inmouse brain tissue, Y79 cells, mouse heart tissue, mouse colon tissue, human placenta tissue, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14139-1-AP targets ADAM12 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM12 fusion protein Ag5206
Full Name ADAM metallopeptidase domain 12
Calculated molecular weight 100 kDa
Observed molecular weight 90-100 kDa, 70-80 kDa
GenBank accession numberBC060804
Gene symbol ADAM12
Gene ID (NCBI) 8038
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.

Protocols

Product Specific Protocols
WB protocol for ADAM12 antibody 14139-1-APDownload protocol
IHC protocol for ADAM12 antibody 14139-1-APDownload protocol
IF protocol for ADAM12 antibody 14139-1-APDownload protocol
IP protocol for ADAM12 antibody 14139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Mol Med

ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.

Authors - Wei Li
humanWB

Nucleic Acids Res

Genetic screens reveal mechanisms for the transcriptional regulation of tissue-specific genes in normal cells and tumors.

Authors - Ikrame Naciri
mouseIHC

Calcif Tissue Int

Involvement of ADAM12 in Chondrocyte Differentiation by Regulation of TGF-β1-Induced IGF-1 and RUNX-2 Expressions.

Authors - Masahiro Horita
  • KO Validated
humanWB

J Proteome Res

SILAC Quantitative Proteomics and Biochemical Analyses Reveal a Novel Molecular Mechanism by which ADAM12S Promotes the Proliferation, Migration, and Invasion of Small Cell Lung Cancer Cells through Upregulating Hexokinase 1.

Authors - Qianqian Duan
  • KD Validated

Reprod Fertil Dev

Soluble Delta-like ligand 1 alters human endometrial epithelial cell adhesive capacity.

Authors - Michelle Van Sinderen
mouseWB, IHC, IF

Biomed Pharmacother

ADAM12 induces EMT and promotes cell migration, invasion and proliferation in pituitary adenomas via EGFR/ERK signaling pathway.

Authors - Junwen Wang
  • KD Validated