ADAMTS4 Polyclonal antibody

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  WB analysis using 11865-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11865-1-AP (ADAMTS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 11865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11865-1-AP (ADAMTS4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 11865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11865-1-AP (ADAMTS4 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 11865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11865-1-AP (ADAMTS4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11865-1-AP (ADAMTS4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADAMTS4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11865-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

A disintegrin and metalloproteinase with thrombospondin motifs 4, ADAM TS 4, ADAM TS4, ADAMTS 4, ADAM-TS 4

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human liver tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

11865-1-AP targets ADAMTS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2443

  Product name: Recombinant human ADAMTS4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2-339 aa of BC030812

  Sequence: SQTGSHPGRGLAGRWLWGAQPCLLLPIVPLSWLVWLLLLLLASLLPSARLASPLPREEEIVFPEKLNGSVLPGSGAPARLLCRLQAFGETLLLELEQDSGVQVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALLGVLQYRGAELHLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPLGSPSPRPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSLVVTRLVILGSGEEGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTRQVRPQSAPQAMHCTILRSATT

• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 4
• Calculated Molecular Weight: 837 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC030812
• Gene Symbol: ADAMTS4
• Gene ID (NCBI): 9507
• RRID: AB_2877799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75173
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat,human	WB	Adv Sci (Weinh); Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis; Authors - Yizhen Huang
mouse	WB	Adv Sci (Weinh); Inhibition of Heat Shock Protein 90β by Catalpol: A Potential Therapeutic Approach for Alleviating Inflammation-Induced Cartilage Injuries in Osteoarthritis; Authors - Zhenwei Zhou
rat	WB	Acta Biomater; ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration; Authors - Tianhui Zhang
pig	WB,IHC	Bone Joint Res; Suramin attenuates intervertebral disc degeneration by inhibiting NF-κB signalling pathway.; Authors - Zi-Miao Liu
mouse	WB	Mol Med Rep; Acetyl zingerone ameliorates osteoarthritis by inhibiting chondrocyte programmed cell death; Authors - Xu Chen
human	WB	Inflammation; Simvastatin Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation by Suppressing the NF-kB and MAPK Pathways in Nucleus Pulposus Cells.; Authors - Ji Tu