Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human liver tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 18 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
11865-1-AP targets ADAMTS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ADAMTS4 fusion protein Ag2443 Predict reactive species |
| Full Name | ADAM metallopeptidase with thrombospondin type 1 motif, 4 |
| Calculated Molecular Weight | 837 aa, 90 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC030812 |
| Gene Symbol | ADAMTS4 |
| Gene ID (NCBI) | 9507 |
| RRID | AB_2877799 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75173 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMTS4 antibody 11865-1-AP | Download protocol |
| IHC protocol for ADAMTS4 antibody 11865-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Biomater ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration | ||
Bone Joint Res Suramin attenuates intervertebral disc degeneration by inhibiting NF-κB signalling pathway. | ||
Mol Med Rep Acetyl zingerone ameliorates osteoarthritis by inhibiting chondrocyte programmed cell death | ||
Inflammation Simvastatin Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation by Suppressing the NF-kB and MAPK Pathways in Nucleus Pulposus Cells. | ||
Clin Exp Pharmacol Physiol Docosahexaenoic acid alleviates the excessive degradation of extracellular matrix in the nucleus pulposus by reducing the content of lncRNA NEAT1 to prevent the progression of intervertebral disc degeneration | ||
Oxid Med Cell Longev The Role of the miR-548au-3p/CA12 Axis in Tracheal Chondrogenesis in Congenital Pulmonary Airway Malformations |
