ADAMTS4 Polyclonal antibody

ADAMTS4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 11865-1-AP


ADAM TS 4, ADAM TS4, ADAMTS 2, ADAMTS 4, ADAMTS4, ADMP 1, Aggrecanase 1, KIAA0688

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11865-1-AP targets ADAMTS4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAMTS4 fusion protein Ag2443
Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 4
Calculated Molecular Weight 837 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC030812
Gene Symbol ADAMTS4
Gene ID (NCBI) 9507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).


Product Specific Protocols
WB protocol for ADAMTS4 antibody 11865-1-APDownload protocol
IHC protocol for ADAMTS4 antibody 11865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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