ADAP1 Polyclonal antibody

ADAP1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13911-1-AP

Synonyms

ADAP1, ArfGAP with dual PH domains 1, CENTA1, Centaurin alpha 1, Cnt a1, GCS1L, p42IP4

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Validation Data Gallery

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  WB analysis of human brain using 13911-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13911-1-AP (ADAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13911-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13911-1-AP (ADAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13911-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13911-1-AP (ADAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13911-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13911-1-AP (ADAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13911-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ADAP1 antibody 13911-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 13911-1-AP

  Immunofluorescent analysis of Hela cells, using ADAP1 antibody 13911-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13911-1-AP targets ADAP1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAP1 fusion protein Ag4898
• Full Name: ArfGAP with dual PH domains 1
• Calculated Molecular Weight: 374 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC033747
• Gene Symbol: ADAP1
• Gene ID (NCBI): 11033
• RRID: AB_2257889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.