ADPGK Polyclonal antibody

ADPGK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15639-1-AP

Synonyms

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, RbBP 35

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Validation Data Gallery

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  WB analysis using 15639-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15639-1-AP (ADPGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15639-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15639-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15639-1-AP

  Immunofluorescent analysis of HepG2 cells using 15639-1-AP (ADPGK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

15639-1-AP targets ADPGK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADPGK fusion protein Ag8105
• Full Name: ADP-dependent glucokinase
• Calculated molecular weight: 497 aa, 54 kDa
• Observed molecular weight: 51 kDa
• GenBank accession number: BC006112
• Gene symbol: ADPGK
• Gene ID (NCBI): 83440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

Publications

Species	Application	Title
human	WB, IF	Front Mol Neurosci; Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.; Authors - Kai Zhang