ADPGK Polyclonal antibody

ADPGK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15639-1-AP


2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, RbBP 35

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15639-1-AP targets ADPGK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADPGK fusion protein Ag8105
Full Name ADP-dependent glucokinase
Calculated Molecular Weight 497 aa, 54 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC006112
Gene Symbol ADPGK
Gene ID (NCBI) 83440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).


Product Specific Protocols
WB protocol for ADPGK antibody 15639-1-APDownload protocol
IHC protocol for ADPGK antibody 15639-1-APDownload protocol
IF protocol for ADPGK antibody 15639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


humanWB, IF

Front Mol Neurosci

Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.

Authors - Kai Zhang