ADPGK Polyclonal antibody

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  WB analysis using 15639-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15639-1-AP (ADPGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15639-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15639-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15639-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ADPGK antibody (15639-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ADPGK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15639-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RbBP 35, PSEC0260, EC:2.7.1.147, ADP-GK, ADP-dependent glucokinase

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

15639-1-AP targets ADPGK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADPGK fusion protein Ag8105
• Full Name: ADP-dependent glucokinase
• Calculated Molecular Weight: 497 aa, 54 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC006112
• Gene Symbol: ADPGK
• Gene ID (NCBI): 83440
• RRID: AB_2224911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRR6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

Publications

Species	Application	Title
human	WB, IF	Front Mol Neurosci; Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.; Authors - Kai Zhang
human	WB	Res Sq; Molecular and clinical characterization of a founder mutation causing G6PC3 deficiency; Authors - Xin Zhen
human	WB	J Clin Immunol; Molecular and Clinical Characterization of a Founder Mutation Causing G6PC3 Deficiency; Authors - Xin Zhen