Name: AFP Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, FC, ELISA
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AFP Antibody WB human brain tissue 14550-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 14550-1-AP(AFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IP Result of anti-AFP (IP:14550-1-AP, 5ug; Detection:14550-1-AP 1:1000) with HepG2 cells lysate 6000ug.

AFP Antibody IHC human liver cancer tissue 14550-1-AP

Immunohistochemical of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 14550-1-AP (AFP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, rat

Positive WB detected in:

human brain tissue, BxPC-3 cells, HepG2 cells, human heart tissue, rat brain tissue, SH-SY5Y cells, SW 1990 cells

Positive IP detected in:

HepG2 cells

Positive IHC detected in:

human liver cancer tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

MCF-7 cells

Recommended dilution:

WB : 1:1000-1:4000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:20-1:200

IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

AFP fusion protein Ag6089

Full name:

alpha-fetoprotein

Calculated molecular weight:

69 kDa

Observed molecular weight:

68-72 kDa

GenBank accession number:

BC027881

Gene ID (NCBI):

174

Gene symbol

AFP

Synonyms

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

Background

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult. So AFP is usually considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker.

Showing 1- [6] of [23] Publications

Author:		Ye Shoudong S
Pubmed ID:		22540008
Journal:		PLoS One
Title:		Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.
Application:		IF

Species:		mouse

Author:		Sone Masayuki M
Pubmed ID:		23041063
Journal:		Am J Pathol
Title:		Recovery of mature hepatocytic phenotype following bile ductular transdifferentiation of rat hepatocytes in vitro.
Application:		FC

Species:		rat

Author:		Zhou Ming M
Pubmed ID:		24134944
Journal:		J Virol Methods
Title:		Long-term maintenance of human fetal hepatocytes and prolonged susceptibility to HBV infection by co-culture with non-parenchymal cells.
Application:		WB

Species:		human

Author:		Mitsuhiro Tsujiwaki
Pubmed ID:		24737165
Journal:		Med Mol Morphol
Title:		Aberrant expression of claudin-4 and -7 in hepatocytes in the cirrhotic human liver.
Application:		IHC

Species:		human

Author:		Ming Zhou
Pubmed ID:		24977316
Journal:		J Virol Methods
Title:		Revival, characterization, and hepatitis B virus infection of cryopreserved human fetal hepatocytes.
Application:		WB

Species:		human

Author:		Ying Yang
Pubmed ID:		23994012
Journal:		Mech Dev
Title:		Stage specific reprogramming of mouse embryo liver cells to a beta cell-like phenotype.
Application:		IF

Species:		mouse

WB Figures

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14550-1-AP(AFP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 14550-1-AP( AFP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

human brain tissue were subjected to SDS PAGE followed by western blot with 14550-1-AP(AFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

AFP Antibody WB human heart tissue 14550-1-AP

human heart tissue were subjected to SDS PAGE followed by western blot with 14550-1-AP(AFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

AFP Antibody WB rat brain tissue 14550-1-AP

rat brain tissue were subjected to SDS PAGE followed by western blot with 14550-1-AP( AFP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

AFP Antibody WB SH-SY5Y cells 14550-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14550-1-AP(AFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

AFP Antibody WB SW 1990 cells 14550-1-AP

SW 1990 cells were subjected to SDS PAGE followed by western blot with 14550-1-AP(AFP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemical of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemical of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:50 (under 40x lens)

AFP Antibody IHC human ovary tumor tissue 14550-1-AP

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP( AFP Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP( AFP Antibody) at dilution of 1:200 (under 40x lens).

IP Figures

IP Result of anti-AFP (IP:14550-1-AP, 5ug; Detection:14550-1-AP 1:1000) with HepG2 cells lysate 6000ug.

IF Figures

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 14550-1-AP (AFP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

AFP Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 14550-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [23] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

AFP Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 14550-1-AP

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Related Primary Antibodies

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background