AFP Polyclonal antibody
AFP Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, Pull-down, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14550-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, BxPC-3 cells, HepG2 cells, human heart tissue, rat brain tissue, SH-SY5Y cells, SW 1990 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IHC | See 22 publications below |
| IF | See 9 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
14550-1-AP targets AFP in WB, Pull-down, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AFP fusion protein Ag6089 |
| Full Name | alpha-fetoprotein |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 68-72 kDa |
| GenBank accession number | BC027881 |
| Gene symbol | AFP |
| Gene ID (NCBI) | 174 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult. So AFP is usually considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AFP antibody 14550-1-AP | Download protocol |
| IHC protocol for AFP antibody 14550-1-AP | Download protocol |
| IF protocol for AFP antibody 14550-1-AP | Download protocol |
| IP protocol for AFP antibody 14550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Breakdown of adaptive immunotolerance induces hepatocellular carcinoma in HBsAg-tg mice. | ||
Theranostics RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog. | ||
Am J Pathol TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma. | ||
Hepatobiliary Pancreat Dis Int Au@SiO2@CuInS2-ZnS/Anti-AFP fluorescent probe improves HCC cell labeling. | ||
World J Gastroenterol Signal transducer and activator of transcription 3 promotes the Warburg effect possibly by inducing pyruvate kinase M2 phosphorylation in liver precancerous lesions. | ||
Cancer Manag Res Peri-tumor fibroblasts promote tumorigenesis and metastasis of hepatocellular carcinoma via Interleukin6/STAT3 signaling pathway. |
