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AFP Antibody 27 Publications

Rabbit Polyclonal| Catalog number: 14550-1-AP

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Con: 62 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

AFP Antibody WB human brain tissue 14550-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 14550-1-AP(AFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

AFP Antibody IP HepG2 cells 14550-1-AP

IP Result of anti-AFP (IP:14550-1-AP, 5ug; Detection:14550-1-AP 1:1000) with HepG2 cells lysate 6000ug.

AFP Antibody IHC human liver cancer tissue 14550-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:50 (under 10x lens)

AFP Antibody IHC human liver cancer tissue 14550-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP(AFP antibody) at dilution of 1:50 (under 40x lens)

AFP Antibody IF MCF-7 cells 14550-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 14550-1-AP (AFP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
human brain tissue, BxPC-3 cells, HepG2 cells, human heart tissue, rat brain tissue, SH-SY5Y cells, SW 1990 cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human liver cancer tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AFP fusion protein Ag6089
Full name:
alpha-fetoprotein
Calculated molecular weight:
69 kDa
Observed molecular weight:
68-72 kDa
GenBank accession number:
BC027881
Gene ID (NCBI):
174
Gene symbol
AFP
Synonyms
AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

Background

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult. So AFP is usually considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker.


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