AFP Recombinant monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 82982-5-RR (same clone as 82982-5-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 82982-5-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82982-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 82982-5-RR (same clone as 82982-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82982-5-RR (AFP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82982-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82982-5-RR (same clone as 82982-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AFP antibody (82982-5-RR, Clone: 230435A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82982-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82982-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82982-5-RR against Human AFP were performed. The affinity constant is 0.696 nM.

AFP Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 82982-5-PBS

Clone No.230435A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

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Product Information

82982-5-PBS targets AFP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: alpha-fetoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: BC027881
• Gene Symbol: AFP
• Gene ID (NCBI): 174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02771
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)