AGL Polyclonal antibody

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AGL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16582-1-AP Copied!

Publications (8)

Reviews (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher

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  WB analysis using 16582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16582-1-AP (AGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 16582-1-AP

  WB result of AGL antibody (16582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGL transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human heart using 16582-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16582-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16582-1-AP targets AGL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9962

  Product name: Recombinant human AGL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1182-1533 aa of BC078663

  Sequence: MYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

• Full Name: amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
• Calculated Molecular Weight: 1532 aa, 175 kDa
• Observed Molecular Weight: 165 kDa
• GenBank Accession Number: BC078663
• Gene Symbol: AGL
• Gene ID (NCBI): 178
• RRID: AB_2878283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35573
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.

Publications

What published studies show

The AGL Polyclonal antibody (Cat# 16582-1-AP) has 8 citations spanning journals including Nature Communications, Journal of Clinical Investigation, Carbohydrate Polymers, JCI Insight, iScience, PLoS One, and Stem Cell Research. Research fields covered include glycogen metabolism, hepatic gluconeogenesis, glycogen storage disease type III (GSDIII), liver fibrosis, gene therapy, post-exercise metabolic regulation, and induced pluripotent stem cell modeling. All citations used WB as the application, with species tested in human, mouse, and rat.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; Multi-layered transcriptional control of glycogen metabolism coordinates thermogenic remodeling of white adipocytes in male mice.; Authors - Haipeng Fu
mouse	WB	J Clin Invest; A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III; Authors - Antoine Gardin
human,mouse	WB	J Clin Invest; Hepatic glycogen directly regulates gluconeogenesis through an AMPK/CRTC2 axis in mice; Authors - Bichen Zhang
rat	WB	Carbohydr Polym; Liver fibrosis alters the molecular structures of hepatic glycogen; Authors - Yujun Wan
mouse	WB	JCI Insight; Synergism of dual AAV gene therapy and rapamycin rescues GSDIII phenotype in muscle and liver; Authors - Louisa Jauze
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding

Reviews

5.0 out of 5

3 verified customer reviews

What customers say

All three reviews are 5-star. Users consistently report excellent performance for Western Blot at a 1:1000 dilution. One reviewer highlighted its high sensitivity for detecting Agl protein across multiple tissues including heart, diaphragm, and various muscles. Another confirmed reliable results using both HEK293 bulk lysate and purified protein. Overall, customers find this antibody to be a dependable reagent with strong sensitivity and consistent performance across different sample types.

Automatically generated from the 3 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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MALLIKARJUNA (Verified Customer) (11-07-2025)

WORKS GOOD FOR WESTERN

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: liver tissue

Pierre-Romain (Verified Customer) (09-18-2025)

work well

• Applications: Other
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293 bulk and protein purified

Jeremy (Verified Customer) (09-08-2019)

Highly sensitive for the detection of Agl protein in different tissue including Heart, Diaphragm and different muscles

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Diaphragm

AGL Antibody Western Blot, validation (1/1000 dilution) in Diaphragm (Cat no:16582-1-AP)