AGL Polyclonal antibody

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  WB analysis using 16582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16582-1-AP (AGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16582-1-AP

  WB result of AGL antibody (16582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGL transfected HEK-293 cells.

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  IHC staining of human heart using 16582-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16582-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

AGL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16582-1-AP

Publications(6)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

16582-1-AP targets AGL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGL fusion protein Ag9962
• Full Name: amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
• Calculated Molecular Weight: 1532 aa, 175 kDa
• Observed Molecular Weight: 165 kDa
• GenBank Accession Number: BC078663
• Gene Symbol: AGL
• Gene ID (NCBI): 178
• RRID: AB_2878283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35573
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III; Authors - Antoine Gardin
rat	WB	Carbohydr Polym; Liver fibrosis alters the molecular structures of hepatic glycogen; Authors - Yujun Wan
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding
rat	WB	PLoS One; A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise; Authors - Seong Eun Kwak
mouse	WB	JCI Insight; Synergism of dual AAV gene therapy and rapamycin rescues GSDIII phenotype in muscle and liver; Authors - Louisa Jauze
human	WB	Stem Cell Res; Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III; Authors - Lucille Rossiaud

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jeremy (Verified Customer) (09-08-2019)

Highly sensitive for the detection of Agl protein in different tissue including Heart, Diaphragm and different muscles

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Diaphragm

AGL Antibody Western Blot, validation (1/1000 dilution) in Diaphragm (Cat no:16582-1-AP)