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AGL Polyclonal antibody

AGL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


mouse, human, rat





Cat no : 16582-1-AP


AGL, Amylo 1,6 glucosidase, Dextrin 6 alpha D glucosidase, GDE, Glycogen debranching enzyme

Tested Applications

Positive WB detected inJurkat cells, mouse heart tissue, HEK-293 cells, rat heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

16582-1-AP targets AGL in WB, IHC, ELISA applications and shows reactivity with mouse, human, rat samples.

Tested Reactivity mouse, human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGL fusion protein Ag9962
Full Name amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Calculated Molecular Weight 1532 aa, 175 kDa
Observed Molecular Weight 165 kDa
GenBank Accession NumberBC078663
Gene Symbol AGL
Gene ID (NCBI) 178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for AGL antibody 16582-1-APDownload protocol
IHC protocol for AGL antibody 16582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Clin Invest

A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III

Authors - Antoine Gardin

Carbohydr Polym

Liver fibrosis alters the molecular structures of hepatic glycogen

Authors - Yujun Wan


MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.

Authors - Dong-Xue Ding

PLoS One

A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise

Authors - Seong Eun Kwak

Stem Cell Res

Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III

Authors - Lucille Rossiaud


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Jeremy (Verified Customer) (09-08-2019)

Highly sensitive for the detection of Agl protein in different tissue including Heart, Diaphragm and different muscles

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Diaphragm
AGL Antibody Western Blot, validation (1/1000 dilution) in Diaphragm (Cat no:16582-1-AP)