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  • KD/KO Validated

AGL Polyclonal antibody

AGL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16582-1-AP

Synonyms

AGL, Amylo 1,6 glucosidase, Dextrin 6 alpha D glucosidase, GDE, Glycogen debranching enzyme



Tested Applications

Positive WB detected inJurkat cells, mouse heart tissue, HEK-293 cells, rat heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

16582-1-AP targets AGL in WB, IHC, ELISA applications and shows reactivity with mouse, human, rat samples.

Tested Reactivity mouse, human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGL fusion protein Ag9962
Full Name amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Calculated Molecular Weight 1532 aa, 175 kDa
Observed Molecular Weight 165 kDa
GenBank Accession NumberBC078663
Gene Symbol AGL
Gene ID (NCBI) 178
RRIDAB_2878283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGL antibody 16582-1-APDownload protocol
IHC protocol for AGL antibody 16582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III

Authors - Antoine Gardin
ratWB

Carbohydr Polym

Liver fibrosis alters the molecular structures of hepatic glycogen

Authors - Yujun Wan
humanWB

iScience

MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.

Authors - Dong-Xue Ding
humanWB

Stem Cell Res

Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III

Authors - Lucille Rossiaud

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jeremy (Verified Customer) (09-08-2019)

Highly sensitive for the detection of Agl protein in different tissue including Heart, Diaphragm and different muscles

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Diaphragm
AGL Antibody Western Blot, validation (1/1000 dilution) in Diaphragm (Cat no:16582-1-AP)