AGL Polyclonal antibody

• Featured Product
• KD/KO Validated

AGL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 16582-1-AP

Synonyms

AGL, Amylo 1,6 glucosidase, Dextrin 6 alpha D glucosidase, GDE, Glycogen debranching enzyme

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Validation Data Gallery

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  WB analysis using 16582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16582-1-AP (AGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16582-1-AP

  WB result of AGL antibody (16582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGL transfected HEK-293 cells.

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  WB analysis of mouse heart using 16582-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16582-1-AP (AGL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 16582-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16582-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse heart tissue, HEK-293 cells, rat heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below

Product Information

16582-1-AP targets AGL in WB, IHC, ELISA applications and shows reactivity with mouse, human, rat samples.

• Tested Reactivity: mouse, human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGL fusion protein Ag9962
• Full Name: amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
• Calculated molecular weight: 1532 aa, 175 kDa
• Observed molecular weight: 165 kDa
• GenBank accession number: BC078663
• Gene symbol: AGL
• Gene ID (NCBI): 178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Carbohydr Polym; Liver fibrosis alters the molecular structures of hepatic glycogen; Authors - Yujun Wan
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding
human	WB	Stem Cell Res; Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III; Authors - Lucille Rossiaud

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jeremy (Verified Customer) (09-08-2019)

Highly sensitive for the detection of Agl protein in different tissue including Heart, Diaphragm and different muscles

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Diaphragm

AGL Antibody Western Blot, validation (1/1000 dilution) in Diaphragm (Cat no:16582-1-AP)