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  • KD/KO Validated

AGL Polyclonal antibody

AGL Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16582-1-AP

Synonyms

AGL, Amylo 1,6 glucosidase, Dextrin 6 alpha D glucosidase, GDE, Glycogen debranching enzyme



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16582-1-AP is AGL Fusion Protein expressed in E. coli.

Tested Reactivity mouse, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGL fusion protein Ag9962
Full Name amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Calculated molecular weight 1532 aa, 175 kDa
Observed molecular weight 165 kDa
GenBank accession numberBC078663
Gene symbol AGL
Gene ID (NCBI) 178
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AGL antibody 16582-1-APDownload protocol
IHC protocol for AGL antibody 16582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jeremy (Verified Customer) (09-08-2019)

Highly sensitive for the detection of Agl protein in different tissue including Heart, Diaphragm and different muscles

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Diaphragm
AGL Antibody Western Blot, validation (1/1000 dilution) in Diaphragm (Cat no:16582-1-AP)