Angiotensinogen Polyclonal antibody

Angiotensinogen Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23972-1-AP

Synonyms

AGT, Ang I Angiotensin 2, Ang II Angiotensin 3, Ang III, Angiotensin, Angiotensin I, Angiotensin II, Angiotensin III, Angiotensinogen, ANHU, Des Asp[1] angiotensin II, Serpin A8, SERPINA8



Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 23972-1-AP is Angiotensinogen Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Angiotensinogen fusion protein Ag21121
Full Name angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Calculated molecular weight 485 aa, 53 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC011519
Gene symbol AGT
Gene ID (NCBI) 183
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Protocols

Product Specific Protocols
WB protocol for Angiotensinogen antibody 23972-1-APDownload protocol
IHC protocol for Angiotensinogen antibody 23972-1-APDownload protocol
IF protocol for Angiotensinogen antibody 23972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

JCI Insight

Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits.

Authors - Wen Ding