Validation Data Gallery
|Positive WB detected in
|Positive IHC detected in
|human liver tissue, human hepatocirrhosis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HEK-293 cells, HepG2 cells, L02 cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
23972-1-AP targets Angiotensinogen in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|Angiotensinogen fusion protein Ag21121
|angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
|Calculated molecular weight
|485 aa, 53 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen Affinity purified
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.