AGTR1 Polyclonal antibody

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  WB analysis using 25343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25343-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 25343-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25343-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25343-1-AP (AGTR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25343-1-AP (AGTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

AGTR1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25343-1-AP

Publications(63)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

AT1, Angiotensin II type-1 receptor, Angiotensin II type 1 receptor, AGTR1B, AGTR1A

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Tested Applications

• Positive WB detected in: C2C12 cells, PC-12 cells, mouse kidney tissue, mouse heart tissue, HuH-7 cells, HEK-293 cells
• Positive IHC detected in: mouse heart tissue, human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 51 publications below
• IHC: See 15 publications below
• IF: See 12 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

25343-1-AP targets AGTR1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGTR1 fusion protein Ag14461
• Full Name: angiotensin II receptor, type 1
• Calculated Molecular Weight: 359 aa, 41 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC022447
• Gene Symbol: AGTR1
• Gene ID (NCBI): 185
• RRID: AB_2880037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30556
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, and salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Publications

Species	Application	Title
human,mouse	WB,CoIP	Cell Res; Cartilage oligomeric matrix protein is an endogenous β-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury.; Authors - Yi Fu
human	WB	Adv Sci (Weinh); Transcriptomics-Based Liquid Biopsy for Early Detection of Recurrence in Locally Advanced Gastric Cancer; Authors - Ping'an Ding ; KD Validated
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
human	IF	Biomaterials; Inhibition of tumor-promoting stroma to enforce subsequently targeting AT1R on tumor cells by pathological inspired micelles.; Authors - Yun Zhu
mouse	WB	Redox Biol; Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system.; Authors - Changmeng Cui
mouse,rat	WB,IF	Free Radic Biol Med; Spinal AT1R contributes to neuroinflammation and neuropathic pain via NOX2-dependent redox signaling in microglia; Authors - Wencui Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kazu (Verified Customer) (12-14-2022)

This antibody worked well on mouse optic nerve sections at optimized dilution of 1:100.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Optic nerve

FH

Wu (Verified Customer) (07-16-2021)

Works good for IHC assay

• Applications: Immunohistochemistry
• Cell Tissue Type: glandular lumen

AGTR1 Antibody Immunohistochemistry validation ( dilution) in glandular lumen (Cat no:25343-1-AP)