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  • KD/KO Validated

AGTR1 Polyclonal antibody

AGTR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 25343-1-AP


AG2S, AGTR1, AGTR1A, AGTR1B, Angiotensin II type 1 receptor, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, AT2R1A, AT2R1B, HAT1R, Type 1 angiotensin II receptor

Tested Applications

Positive WB detected inC2C12 cells, PC-12 cells, mouse kidney tissue, mouse heart tissue, HuH-7 cells, HEK-293 cells
Positive IHC detected inmouse heart tissue, human liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25343-1-AP targets AGTR1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGTR1 fusion protein Ag14461
Full Name angiotensin II receptor, type 1
Calculated Molecular Weight 359 aa, 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC022447
Gene Symbol AGTR1
Gene ID (NCBI) 185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, and salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).


Product Specific Protocols
WB protocol for AGTR1 antibody 25343-1-APDownload protocol
IHC protocol for AGTR1 antibody 25343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

Cartilage oligomeric matrix protein is an endogenous β-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury.

Authors - Yi Fu

Aging Cell

Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling

Authors - Ruili Dang


Inhibition of tumor-promoting stroma to enforce subsequently targeting AT1R on tumor cells by pathological inspired micelles.

Authors - Yun Zhu

Redox Biol

Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system.

Authors - Changmeng Cui


A zebrafish screen reveals Renin-angiotensin system inhibitors as neuroprotective via mitochondrial restoration in dopamine neurons.

Authors - Gha-Hyun J Kim
  • KD Validated

Int J Mol Sci

Exercise Normalized the Hippocampal Renin-Angiotensin System and Restored Spatial Memory Function, Neurogenesis, and Blood-Brain Barrier Permeability in the 2K1C-Hypertensive Mouse.

Authors - Ying-Shuang Chang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kazu (Verified Customer) (12-14-2022)

This antibody worked well on mouse optic nerve sections at optimized dilution of 1:100.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Optic nerve

Wu (Verified Customer) (07-16-2021)

Works good for IHC assay

  • Applications: Immunohistochemistry
  • Cell Tissue Type: glandular lumen
AGTR1 Antibody Immunohistochemistry validation ( dilution) in glandular lumen (Cat no:25343-1-AP)