AHNAK Polyclonal antibody

AHNAK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16637-1-AP

Synonyms

AHNAK, AHNAK nucleoprotein, AHNAKRS, Desmoyokin, PM227

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Validation Data Gallery

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  WB analysis using 16637-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 16637-1-AP (AHNAK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16637-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHNAK antibody (16637-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human oesophagus cancer tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below
• FC: See 1 publications below

Product Information

16637-1-AP targets AHNAK in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AHNAK fusion protein Ag9986
• Full Name: AHNAK nucleoprotein
• Calculated Molecular Weight: 629 kDa
• GenBank Accession Number: BC000926
• Gene Symbol: AHNAK
• Gene ID (NCBI): 79026
• RRID: AB_2878291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AHNAK, also known as desmoyokin, is described as a giant scaffold protein based on its large size (629 kDa) and ability to interact with different proteins to form multi-protein complexes. The bulk of the protein is assembled in 128-residue repetitive elements known as the central repeated unit (CRU). Its proposed functions are quite diverse, ranging from a role in the formation of the blood brain barrier, in cell architecture and migration, to the regulation of cardiac channels and muscle membrane repair. AHNAK is differentially expressed in some cancer cell lines. The expression of AHNAK is subsequently localized to the plasma membrane of keratinocytes in human epidermis. AHNAK has been reported in many intracellular locations including the nucleus, cytoplasm and plasma membrane.

Publications

Species	Application	Title
human	IF	Nat Commun; Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.; Authors - Yuta Asano
human	IF	EMBO Rep; Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging; Authors - Monami Ogura
human	FC	Clin Exp Immunol; The AHNAK induces increased IL-6 production in CD4+T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss.; Authors - Liman Li