AHNAK Polyclonal antibody

AHNAK Polyclonal Antibody for FC, IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16637-1-AP

Synonyms

AHNAK, AHNAK nucleoprotein, AHNAKRS, Desmoyokin, PM227



Tested Applications

Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

16637-1-AP targets AHNAK in IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHNAK fusion protein Ag9986
Full Name AHNAK nucleoprotein
Calculated molecular weight 629 kDa
GenBank accession numberBC000926
Gene symbol AHNAK
Gene ID (NCBI) 79026
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AHNAK, also known as desmoyokin, is described as a giant scaffold protein based on its large size (629 kDa) and ability to interact with different proteins to form multi-protein complexes. The bulk of the protein is assembled in 128-residue repetitive elements known as the central repeatedunit (CRU). Its proposed functions are quite diverse, ranging from a role in the formation of the blood brain barrier, in cell architecture and migration, to the regulation of cardiac channels and muscle membrane repair. AHNAK is differentially expressed in some cancer cell lines. The expression of AHNAK is subsequently localized to the plasma membrane of keratinocytes in human epidermis. AHNAK has been reported in many intracellular locations including the nucleus, cytoplasm and plasma membrane.

Protocols

Product Specific Protocols
IHC protocol for AHNAK antibody 16637-1-APDownload protocol
IF protocol for AHNAK antibody 16637-1-APDownload protocol
FC protocol for AHNAK antibody 16637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Authors - Yuta Asano