AHNAK Polyclonal antibody

AHNAK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16637-1-AP

Synonyms

AHNAK, AHNAK nucleoprotein, AHNAKRS, Desmoyokin, PM227



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman oesophagus cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16637-1-AP targets AHNAK in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHNAK fusion protein Ag9986
Full Name AHNAK nucleoprotein
Calculated molecular weight 629 kDa
GenBank accession numberBC000926
Gene symbol AHNAK
Gene ID (NCBI) 79026
RRIDAB_2878291
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AHNAK, also known as desmoyokin, is described as a giant scaffold protein based on its large size (629 kDa) and ability to interact with different proteins to form multi-protein complexes. The bulk of the protein is assembled in 128-residue repetitive elements known as the central repeated unit (CRU). Its proposed functions are quite diverse, ranging from a role in the formation of the blood brain barrier, in cell architecture and migration, to the regulation of cardiac channels and muscle membrane repair. AHNAK is differentially expressed in some cancer cell lines. The expression of AHNAK is subsequently localized to the plasma membrane of keratinocytes in human epidermis. AHNAK has been reported in many intracellular locations including the nucleus, cytoplasm and plasma membrane.

Protocols

Product Specific Protocols
WB protocol for AHNAK antibody 16637-1-APDownload protocol
IHC protocol for AHNAK antibody 16637-1-APDownload protocol
IF protocol for AHNAK antibody 16637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Authors - Yuta Asano
humanIF

EMBO Rep

Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging

Authors - Monami Ogura
humanFC

Clin Exp Immunol

The AHNAK induces increased IL-6 production in CD4+T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss.

Authors - Liman Li