Name: AHR Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
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MCF7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP(AHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

AHR Antibody IHC human brain tissue 17840-1-AP

Immunohistochemical of paraffin-embedded human brain using 17840-1-AP(AHR antibody) at dilution of 1:100 (under 10x lens)

IP Result of anti-AHR (IP:17840-1-AP, 4ug; Detection:17840-1-AP 1:500) with PC-3 cells lysate 1880ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

MCF7 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, PC-3 cells

Positive IP detected in:

PC-3 cells

Positive IHC detected in:

human brain tissue, mouse small intestine tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:20-1:200

Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

AHR fusion protein Ag12193

Full name:

aryl hydrocarbon receptor

Calculated molecular weight:

848aa,96 kDa

Observed molecular weight:

105 kDa

GenBank accession number:

BC070080

Gene ID (NCBI):

196

Gene symbol

AHR

Synonyms

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

Background

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Showing 1- [4] of [4] Publications

Author:		Xin-Chan Feng
Pubmed ID:		25755705
Journal:		Int J Clin Exp Pathol
Title:		Swertianlarin, isolated from Swertia mussotii Franch, increases detoxification enzymes and efflux transporters expression in rats.
Application:		WB

Species:		rat

Author:		Jin Chai
Pubmed ID:		25798860
Journal:		PLoS One
Title:		Hepatic expression of detoxification enzymes is decreased in human obstructive cholestasis due to gallstone biliary obstruction.
Application:		WB

Species:		human

Author:		Jin Chai
Pubmed ID:		25932098
Journal:		Int J Clin Exp Med
Title:		Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury, inflammation, and cholestasis in bile duct-ligated rats.
Application:		WB

Species:		rat

Author:		Qin Liu
Pubmed ID:		28849137
Journal:		Int J Mol Med
Title:		Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes.
Application:		IF

Species:		human

WB Figures

AHR Antibody WB HEK-293 cells 17840-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP(AHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP(AHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP(AHR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

MCF-7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP( AHR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

MCF-7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP( AHR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

AHR Antibody WB NIH/3T3 cells 17840-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP(AHR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

PC-3 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP(AHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human brain using 17840-1-AP(AHR antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human brain using 17840-1-AP(AHR antibody) at dilution of 1:100 (under 40x lens)

AHR Antibody IHC mouse small intestine tissue 17840-1-AP

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Figures

IP Result of anti-AHR (IP:17840-1-AP, 4ug; Detection:17840-1-AP 1:500) with PC-3 cells lysate 1880ug.

AHR Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 17840-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [4] of [4] Publications

WB Figures

IHC Figures

IP Figures

AHR Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 17840-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background