AHR Polyclonal antibody

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  WB analysis using 17840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17840-1-AP

  WB result of AHR antibody (17840-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHR transfected HepG2 cells.

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  IP experiment of PC-3 using 17840-1-AP

  IP result of anti-AHR (IP:17840-1-AP, 4ug; Detection:17840-1-AP 1:500) with PC-3 cells lysate 1880ug.

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  IHC staining of human lung cancer using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17840-1-AP (AHR antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17840-1-AP (AHR antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse small intestine using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

AHR Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17840-1-AP

Publications(50)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human lung cancer tissue, mouse small intestine tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 44 publications below
• IHC: See 11 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

17840-1-AP targets AHR in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12193

  Product name: Recombinant human AHR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-387 aa of BC070080

  Sequence: MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQGKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGSGYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLT

• Full Name: aryl hydrocarbon receptor
• Calculated Molecular Weight: 848 aa, 96 kDa
• Observed Molecular Weight: 105-110 kDa
• GenBank Accession Number: BC070080
• Gene Symbol: AHR
• Gene ID (NCBI): 196
• RRID: AB_2226163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35869
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk
human	WB	J Immunother Cancer; GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer.; Authors - Jin-Li Wei ; KD Validated
human,mouse	WB	Theranostics; Activating AhR alleviates cognitive deficits of Alzheimer's disease model mice by upregulating endogenous Aβ catabolic enzyme Neprilysin.; Authors - Cheng Qian ; KD Validated
mouse	WB	Br J Pharmacol; AhR-STAT3-Hmox1/Ptgs2 signaling pathway may restrict ferroptosis and improve hMSC accumulation and efficacy in mice liver; Authors - Li Han
mouse	WB	mSystems; Impaired Intestinal Akkermansia muciniphila and Aryl Hydrocarbon Receptor Ligands Contribute to Nonalcoholic Fatty Liver Disease in Mice.; Authors - Zunji Shi
mouse	WB,IHC,IF	J Invest Dermatol; Aryl Hydrocarbon Receptor in Cutaneous Vascular Endothelial Cells Restricts Psoriasis Development by Negatively Regulating Neutrophil Recruitment.; Authors - Zhenlai Zhu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (10-03-2024)

Used this antibody for colon carcinoma cell line and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Hala (Verified Customer) (04-13-2021)

works good!

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2, U937