AHR Polyclonal antibody

• Featured Product
• KD/KO Validated

AHR Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

30

Reviews (1)

Cat no : 17840-1-AP

Synonyms

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 17840-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17840-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17840-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17840-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 17840-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17840-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17840-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17840-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 17840-1-AP

  IP Result of anti-AHR (IP:17840-1-AP, 4ug; Detection:17840-1-AP 1:500) with PC-3 cells lysate 1880ug.

• 

  IHC staining of human lung cancer using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17840-1-AP (AHR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17840-1-AP (AHR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF7 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human lung cancer tissue, mouse small intestine tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 27 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

17840-1-AP targets AHR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AHR fusion protein Ag12193
• Full Name: aryl hydrocarbon receptor
• Calculated molecular weight: 848 aa, 96 kDa
• Observed molecular weight: 105-110 kDa
• GenBank accession number: BC070080
• Gene symbol: AHR
• Gene ID (NCBI): 196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Publications

Species	Application	Title
rat	WB	Cell Death Dis; Tetrandrine enhances the ubiquitination and degradation of Syk through an AhR-c-src-c-Cbl pathway and consequently inhibits osteoclastogenesis and bone destruction in arthritis.; Authors - Yugai Jia
rat	IHC	Front Cell Dev Biol; Human Umbilical Mesenchymal Stem Cells Display Therapeutic Potential in Rheumatoid Arthritis by Regulating Interactions Between Immunity and Gut Microbiota via the Aryl Hydrocarbon Receptor.; Authors - Xiaoya Li
mouse	WB	Cell Cycle; LncRNA GAS5 inhibits NLRP3 inflammasome activation-mediated pyroptosis in diabetic cardiomyopathy by targeting miR-34b-3p/AHR.; Authors - Yingying Xu
human,mouse	WB,IF	Signal Transduct Target Ther; Both IDO1 and TDO contribute to the malignancy of gliomas via the Kyn-AhR-AQP4 signaling pathway.; Authors - Lisha Du
human	WB	J Immunol Res; ZNF165 Is Involved in the Regulation of Immune Microenvironment and Promoting the Proliferation and Migration of Hepatocellular Carcinoma by AhR/CYP1A1.; Authors - Peng Liu
rat	WB	Int J Clin Exp Med; Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury, inflammation, and cholestasis in bile duct-ligated rats.; Authors - Jin Chai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (04-13-2021)

works good!

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2, U937

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