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AHR Polyclonal antibody

AHR Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse and More (1)





Cat no : 28727-1-AP


Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

Tested Applications

Positive WB detected inA431 cells, HepG2 cells
Positive IP detected inPC-3 cells
Positive IHC detected inmouse small intestine tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28727-1-AP targets AHR in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHR fusion protein Ag28935
Full Name aryl hydrocarbon receptor
Calculated molecular weight 848 aa, 96 kDa
Observed molecular weight105-110 kDa
GenBank accession numberBC070080
Gene symbol AHR
Gene ID (NCBI) 196
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)


Product Specific Protocols
WB protocol for AHR antibody 28727-1-APDownload protocol
IHC protocol for AHR antibody 28727-1-APDownload protocol
IF protocol for AHR antibody 28727-1-APDownload protocol
IP protocol for AHR antibody 28727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int Immunopharmacol

Paeoniflorin-6'-o-benzene sulfonate ameliorates the progression of adjuvant-induced arthritis by inhibiting the interaction between Ahr and GRK2 of fibroblast-like synoviocytes.

Authors - Bin-Jie Zhang

J Ethnopharmacol

Ginsenoside Rg1 protects mice against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced liver injury by inhibiting CYP1A1 through the aryl hydrocarbon receptor.

Authors - Han Li

Toxicol Res (Camb)

High-fat diet and alcohol induced-mice could cause colonic injury through molecular mechanisms of endogenous toxins

Authors - Shumin Zhu

Cell Prolif

Porphyromonas gingivalis infection promotes inflammation via inhibition of the AhR signalling pathway in periodontitis

Authors - Ting Hao

Oncol Lett

Fenofibrate attenuates the cytotoxic effect of cisplatin on lung cancer cells by enhancing the antioxidant defense system in vitro

Authors - Mariko Kogami

Poult Sci

Bacillus subtilis KC1 prevents Mycoplasma gallisepticum-induced lung injury by enhancing intestinal Bifidobacterium animalis and regulating indole metabolism in chickens

Authors - Xueping Chen