AIDA Monoclonal antibody, PBS Only

AIDA Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11A1

Cat no : 68153-1-PBS

Synonyms

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

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Validation Data Gallery

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  WB analysis using 68153-1-Ig (same clone as 68153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68153-1-Ig (same clone as 68153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68153-1-Ig (same clone as 68153-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 68153-1-Ig (same clone as 68153-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AIDA antibody (68153-1-Ig, Clone: 1D11A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68153-1-PBS targets AIDA in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AIDA fusion protein Ag20597
• Full Name: axin interactor, dorsalization associated
• Calculated Molecular Weight: 306 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC015535
• Gene Symbol: AIDA
• Gene ID (NCBI): 64853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.