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- KD/KO Validated
AIFM2/ FSP1 Polyclonal antibody
AIFM2/ FSP1 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 20886-1-AP
|Positive WB detected in||mouse kidney tissue, HeLa cells, rat liver tissue, HepG2 cells, PC-12 ceslls|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20886-1-AP targets AIFM2/ FSP1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig, duck|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AIFM2/ FSP1 fusion protein Ag13412|
|Full Name||apoptosis-inducing factor, mitochondrion-associated, 2|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||41 kDa|
|GenBank accession number||BC023601|
|Gene ID (NCBI)||84883|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression
The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.
A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.
Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.
A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers.
Proc Natl Acad Sci U S A
HOIP modulates the stability of GPx4 by linear ubiquitination