AIFM2/ FSP1 Polyclonal antibody

AIFM2/ FSP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

46

Cat no : 20886-1-AP

Synonyms

AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, ferroptosis suppressor protein 1, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2

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Validation Data Gallery

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  WB analysis using 20886-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 20886-1-AP

  IP Result of anti-AIFM2 (IP:20886-1-AP, 4ug; Detection:20886-1-AP 1:300) with L02 cells lysate 1800ug.

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  IHC staining of human liver using 20886-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 20886-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 20886-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 20886-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 37 publications below
• IHC: See 9 publications below
• IF: See 7 publications below

Product Information

The immunogen of 20886-1-AP is AIFM2/ FSP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AIFM2/ FSP1 fusion protein Ag13412
• Full Name: apoptosis-inducing factor, mitochondrion-associated, 2
• Calculated molecular weight: 41 kDa
• Observed molecular weight: 41 kDa
• GenBank accession number: BC023601
• Gene symbol: AIFM2
• Gene ID (NCBI): 84883
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

Publications

Species	Application	Title
human	WB	Nature; The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.; Authors - Kirill Bersuker
mouse	WB	Nat Commun; A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.; Authors - Fan Yao
mouse	IHC,IF	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu
human	WB	Nat Commun; A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers.; Authors - Pranavi Koppula
mouse	WB	Proc Natl Acad Sci U S A; HOIP modulates the stability of GPx4 by linear ubiquitination; Authors - Kangyun Dong
human	WB,IHC	Oncogene; The microRNA-3622 family at the 8p21 locus exerts oncogenic effects by regulating the p53-downstream gene network in prostate cancer progression.; Authors - Yue Zhang