AIFM2/ FSP1 Polyclonal antibody

AIFM2/ FSP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20886-1-AP

Synonyms

AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, ferroptosis suppressor protein 1, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2



Tested Applications

Positive WB detected inmouse kidney tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20886-1-AP is AIFM2/ FSP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, mosue
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIFM2/ FSP1 fusion protein Ag13412
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Calculated molecular weight 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC023601
Gene symbol AIFM2
Gene ID (NCBI) 84883
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

Protocols

Product Specific Protocols
WB protocol for AIFM2/ FSP1 antibody 20886-1-APDownload protocol
IHC protocol for AIFM2/ FSP1 antibody 20886-1-APDownload protocol
IP protocol for AIFM2/ FSP1 antibody 20886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

HCAR1/MCT1 Regulates Tumor Ferroptosis through the Lactate-Mediated AMPK-SCD1 Activity and Its Therapeutic Implications.

Authors - Youbo Zhao
humanWB

Cell Death Dis

DHA exhibits synergistic therapeutic efficacy with cisplatin to induce ferroptosis in pancreatic ductal adenocarcinoma via modulation of iron metabolism.

Authors - Jing Du
humanWB,IHC

Oxid Med Cell Longev

Comprehensive Analysis of Ferroptosis-Related Markers for the Clinical and Biological Value in Gastric Cancer.

Authors - Yanfei Shao
humanWB

Cell Death Dis

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.

Authors - Shicheng Sun
human,mouseWB,IHC

Cell Death Dis

Plasma-activated medium induces ferroptosis by depleting FSP1 in human lung cancer cells.

Authors - Ara Jo
mouseWB

Int J Oral Sci

Porphyromonas gingivalis disrupts vascular endothelial homeostasis in a TLR-NF-κB axis dependent manner.

Authors - Mengru Xie