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AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3E12A8

Cat no : 66902-1-Ig

Synonyms

PYHIN4, Interferon-inducible protein AIM2, 3E12A8



Tested Applications

Positive WB detected inRAW 264.7 cells, rabbit spleen tissue, human heart tissue, Sp2/0 cells
Positive IHC detected inhuman urothelial carcinoma tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66902-1-Ig targets AIM2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIM2 fusion protein Ag14607
Full Name absent in melanoma 2
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight 37-39 and 53 kDa
GenBank Accession NumberBC010940
Gene Symbol AIM2
Gene ID (NCBI) 9447
RRIDAB_2882230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

Protocols

Product Specific Protocols
WB protocol for AIM2 antibody 66902-1-IgDownload protocol
IHC protocol for AIM2 antibody 66902-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Gut

STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis.

Authors - Ruby E Dawson
humanWB

Theranostics

Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy

Authors - Qingyu Liang
humanWB

Front Oncol

PKIB involved in the metastasis and survival of osteosarcoma

Authors - Rongxue Wan
mouseWB

Cell Commun Signal

Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia.

Authors - Dong-Ming Wu
mouseWB

Sci Total Environ

Cadmium exposure induces pyroptosis in testicular tissue by increasing oxidative stress and activating the AIM2 inflammasome pathway.

Authors - Jinzhao Zhou
mouseWB,IF

EMBO J

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses

Authors - Hao Liu
  • KD Validated