Validation Data Gallery
|Positive WB detected in
|RAW 264.7 cells, rabbit spleen tissue, human heart tissue, Sp2/0 cells
|Positive IHC detected in
|human urothelial carcinoma tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:500-1:2000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66902-1-Ig targets AIM2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.
|Human, mouse, rat, rabbit
|Host / Isotype
|Mouse / IgG1
|AIM2 fusion protein Ag14607
|absent in melanoma 2
|Calculated molecular weight
|343 aa, 39 kDa
|Observed molecular weight
|37-39 and 53 kDa
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]
STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis.
Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy
PKIB involved in the metastasis and survival of osteosarcoma
Cell Commun Signal
Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia.
Sci Total Environ
Cadmium exposure induces pyroptosis in testicular tissue by increasing oxidative stress and activating the AIM2 inflammasome pathway.
Prohibitin 1 regulates mtDNA release and downstream inflammatory responses