AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit and More (1)

Applications

WB, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3E12A8

Cat no : 66902-1-Ig

Synonyms

absent in melanoma 2, AIM2, PYHIN4



Tested Applications

Positive WB detected inRAW 264.7 cells, rabbit spleen tissue, human heart tissue, Sp2/0 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66902-1-Ig targets AIM2 in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIM2 fusion protein Ag14607
Full Name absent in melanoma 2
Calculated molecular weight 343 aa, 39 kDa
Observed molecular weight 37-39 and 53 kDa
GenBank accession numberBC010940
Gene symbol AIM2
Gene ID (NCBI) 9447
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

Protocols

Product Specific Protocols
WB protocol for AIM2 antibody 66902-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Total Environ

Cadmium exposure induces pyroptosis in testicular tissue by increasing oxidative stress and activating the AIM2 inflammasome pathway.

Authors - Jinzhao Zhou
mouseWB

Cell Commun Signal

Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia.

Authors - Dong-Ming Wu
humanWB

Theranostics

Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy

Authors - Qingyu Liang
humanCoIP

Gut

STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis.

Authors - Ruby E Dawson