AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit and More (1)

Applications

WB, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3E12A8

Cat no : 66902-1-Ig

Synonyms

absent in melanoma 2, AIM2, PYHIN4



Tested Applications

Positive WB detected inRAW 264.7 cells, rabbit spleen tissue, human heart tissue, Sp2/0 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

CoIPSee 1 publications below

Product Information

66902-1-Ig targets AIM2 in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIM2 fusion protein Ag14607
Full Name absent in melanoma 2
Calculated molecular weight 343 aa, 39 kDa
Observed molecular weight 37-39 and 53 kDa
GenBank accession numberBC010940
Gene symbol AIM2
Gene ID (NCBI) 9447
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

Protocols

Product Specific Protocols
WB protocol for AIM2 antibody 66902-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Gut

STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis.

Authors - Ruby E Dawson