AIP/ARA9 Polyclonal antibody

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18176-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18176-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 18176-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug AIP/ARA9 Polyclonal antibody (18176-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

AIP/ARA9 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 18176-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

AIP, ARA9, XAP 2, XAP2, AH receptor-interacting protein

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

18176-1-AP targets AIP/ARA9 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12869

  Product name: Recombinant human AIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-330 aa of BC104797

  Sequence: MADIIARLREDGIQKRVIQEGRGELPDFQDGTKATFHYRTLHSDDEGTVLDDSRARGKPMELIIGKKFKLPVWETIVCTMREGEIAQFLCDIKHVVLYPLVAKSLRNIAVGKDPLEGQRHCCGVAQMREHSSLGHADLDALQQNPQPLIFHMEMLKVESPGTYQQDPWAMTDEEKAKAVPLIHQEGNRLYREGHVKEAAAKYYDAIACLKNLQMKEQPGSPEWIQLDKQITPLLLNYCQCKLVVEEYYEVLDHCSSILNKYDDNVKAYFKRGKAHAAVWNAQEAQADFAKVLELDPALAPVVSRELRALEARIRQKDEEDKARFRGIFSH

• Full Name: aryl hydrocarbon receptor interacting protein
• Calculated Molecular Weight: 330 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC104797
• Gene Symbol: AIP
• Gene ID (NCBI): 9049
• RRID: AB_11182727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00170
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IP	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk
rat	WB,IHC	Heliyon; PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats; Authors - Wei Zhang