AKAP13 Polyclonal antibody

AKAP13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27947-1-AP

Synonyms

A kinase anchor protein 13, AKAP 13, AKAP Lbc, AKAP13, ARHGEF13, BRX, c lbc, HA 3, Ht31, LBC, LBC oncogene, Lymphoid blast crisis oncogene, p47, PROTO LB, PROTO LBC

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Validation Data Gallery

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  WB analysis using 27947-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27947-1-AP (AKAP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27947-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27947-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27947-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27947-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27947-1-AP targets AKAP13 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKAP13 fusion protein Ag27486
• Full Name: A kinase (PRKA) anchor protein 13
• Calculated Molecular Weight: 308 kDa
• GenBank Accession Number: NM_001270546
• Gene Symbol: AKAP13
• Gene ID (NCBI): 11214
• RRID: AB_2881017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.