AKR1B10 Polyclonal antibody

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  WB analysis using 18252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18252-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18252-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18252-1-AP

  IP result of anti-AKR1B10 (IP:18252-1-AP, 4ug; Detection:18252-1-AP 1:4000) with HepG2 cells lysate 2000 ug.

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  IHC staining of mouse stomach using 18252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18252-1-AP (AKR1B10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 18252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18252-1-AP (AKR1B10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AKR1B10 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 18252-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

AKR1B 10, AKR1B11, AKR1B12, Aldo-keto reductase family 1 member B10, Aldose reductase-like

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Tested Applications

• Positive WB detected in: A549 cells, mouse liver tissue, HepG2 cells, A431 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

18252-1-AP targets AKR1B10 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13042

  Product name: Recombinant human AKR1B10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-316 aa of BC008837

  Sequence: MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFDAEY

• Full Name: aldo-keto reductase family 1, member B10 (aldose reductase)
• Calculated Molecular Weight: 316 aa, 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC008837
• Gene Symbol: AKR1B10
• Gene ID (NCBI): 57016
• RRID: AB_2878524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60218
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldo-keto reductase family 1 member B10 (AKR1B10) is a member of the AKR1B subfamily. It is mainly found in cytoplasm, and it is typically expressed in the stomach and intestines. Given that its expression is low or absent in other tissues, AKR1B10 is a potential diagnostic and therapeutic biomarker for various digestive system diseases.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Pharmacol Exp Ther; Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.; Authors - Ramon Martinez
mouse,human	WB	J Ethnopharmacol; Berberine directly targets AKR1B10 protein to modulate lipid and glucose metabolism disorders in NAFLD; Authors - Sa Yang ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In tissues, we detected bands at different positions, but in cancer cell lines, the bands appeared notably clearer. The optimal concentration was determined to be higher, specifically at 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines and tumor tissues

AKR1B10 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines and tumor tissues (Cat no:18252-1-AP)