AKT Polyclonal antibody
AKT Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, Mouse and More (1)
WB, IHC, ELISA
Cat no : 55230-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55230-1-AP targets AKT in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples.
|Tested Reactivity||human, Mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||v-akt murine thymoma viral oncogene homolog 1|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||56-62 kDa|
|GenBank accession number||NM_001014431|
|Gene ID (NCBI)||207|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
AKT1, also named as PKB and RAC, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily. It plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. AKT1 promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. It plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. AKT1 mediates the antiapoptotic effects of IGF-I. (PMID:16139227)This antibody is specific to AKT1. It has no cross reaction to AKT2 and AKT3.
|Product Specific Protocols|
|WB protocol for AKT antibody 55230-1-AP||Download protocol|
|IHC protocol for AKT antibody 55230-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Med Rep
Matrine inhibits the invasive and migratory properties of human hepatocellular carcinoma by regulating epithelial‑mesenchymal transition.
The Intracellular Interaction of Porcine β-Defensin 2 with VASH1 Alleviates Inflammation via Akt Signaling Pathway.
Int J Mol Sci
Extracellular Calcium-Induced Calcium Transient Regulating the Proliferation of Osteoblasts through Glycolysis Metabolism Pathways
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution and 1:100 in IF
Tanusree (Verified Customer) (08-14-2019)
The antibody works good in mouse brain tissue in western blotting.