AKT1 Polyclonal antibody

AKT1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55230-1-AP

Synonyms

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha



Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55230-1-AP targets AKT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 56-62 kDa
GenBank accession numberNM_001014431
Gene symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

AKT1, also named as PKB and RAC, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily. It plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. AKT1 promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. It plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. AKT1 mediates the antiapoptotic effects of IGF-I. (PMID:16139227)This antibody is specific to AKT1. It has no cross reaction to AKT2 and AKT3.

Protocols

Product Specific Protocols
WB protocol for AKT1 antibody 55230-1-APDownload protocol
IHC protocol for AKT1 antibody 55230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution and 1:100 in IF

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Tanusree (Verified Customer) (08-14-2019)

The antibody works good in mouse brain tissue in western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain