AKTIP Recombinant monoclonal antibody

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  WB analysis using 86988-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86988-1-RR (AKTIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86988-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86988-1-RR against Human AKTIP were performed. The affinity constant is below 1 pM.

AKTIP Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86988-1-RR

Clone No.251957F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AKT-interacting protein, Ft1, FTS

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, MCF-7 cells, HepG2 cells, U-251 cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86988-1-RR targets AKTIP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5816

  Product name: Recombinant human AKTIP protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 2-292 aa of NM_022476.4

  Sequence: NPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT

• Full Name: AKT interacting protein
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: NM_022476.4
• Gene Symbol: AKTIP
• Gene ID (NCBI): 64400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H8T0-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKTIP is a telomeric protein with the property of being enriched at the nuclear lamina. AKTIP has similarity with the tumor susceptibility gene TSG101. AKTIP deficiency generates genome instability and, in p53−/− mice, the reduction of the mouse counterpart of AKTIP induces the exacerbation of lymphomas.