Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, mouse brain tissue, HepG2 cells, MCF-7 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17719-1-AP targets P5CS in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12100 Product name: Recombinant human ALDH18A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-362 aa of BC106054 Sequence: MLSQVYRCGFQPFNQHLLPWVKCTTVFRSHCIQPSVIRHVRSWSNIPFITVPLSRTHGKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGIKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPT Predict reactive species |
| Full Name | aldehyde dehydrogenase 18 family, member A1 |
| Calculated Molecular Weight | 795 aa, 87 kDa |
| Observed Molecular Weight | 87 kDa |
| GenBank Accession Number | BC106054 |
| Gene Symbol | P5CS |
| Gene ID (NCBI) | 5832 |
| RRID | AB_2223896 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54886 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS and PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for P5CS antibody 17719-1-AP | Download protocol |
| IHC protocol for P5CS antibody 17719-1-AP | Download protocol |
| IP protocol for P5CS antibody 17719-1-AP | Download protocol |
| WB protocol for P5CS antibody 17719-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-P5CS (ALDH18A1) antibody, catalog number 17719-1-AP, has 40 citations. Publications appear in high-impact journals including Nature, Nature Metabolism, Nature Communications, Cell Death & Differentiation, PNAS, Oncogene, FASEB Journal, and Journal of Biological Chemistry, among others. Research fields span proline/glutamine metabolism, ferroptosis, cancer metabolic reprogramming, mitochondrial biology, pulmonary fibrosis, neurodegeneration, and immune-metabolic interactions, with applications primarily in WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Nature Cellular ATP demand creates metabolically distinct subpopulations of mitochondria
| ||
Nat Metab Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.
| ||
Nat Metab Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix. | ||
Nat Metab Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells
| ||
Autophagy Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis. |
Reviews
What customers say
The single review rates the antibody 4 out of 5 stars for Western Blot on human kidney cancer cells (RCC4). The user lysed cells at 95°C in SDS-loading buffer, ran a 4–20% denaturing SDS-PAGE, and blotted to a nitrocellulose membrane. Detection used the primary antibody at 1:1,000 in blocking buffer (PBS, 5% milk, 0.1% Tween-20), a secondary at 1:10,000, and ECL reagents, yielding a clear signal. Overall, the reviewer found the antibody effective for WB under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kristian (Verified Customer) (02-05-2020) | The cells were collected, then lyzed five minutes at 95C in SDS-loading buffer.The lysate was run on a 4-20% denaturing SDS-PAGE, then blotted unto a nitrocellulose membrane. The protein was detected using primary antibody diluted 1:1,000 in blotto (PBS, 5% milk, 0.1% Tween-20), then secondary in a 1:10,000 dilution in blotto.Detected using ECL reagents.
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