P5CS Polyclonal antibody

• Featured Product
• KD/KO Validated

P5CS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 17719-1-AP

Synonyms

ALDH18A1, Aldehyde dehydrogenase family 18 member A1, GK, Gamma-glutamyl phosphate reductase, Gamma-glutamyl kinase

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Validation Data Gallery

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  WB analysis using 17719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17719-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17719-1-AP

  IP result of anti-P5CS (IP:17719-1-AP, 4ug; Detection:17719-1-AP 1:1000) with mouse brain tissue lysate 1600 ug.

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  IHC staining of human breast cancer using 17719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17719-1-AP (P5CS antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17719-1-AP (P5CS antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 17719-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using P5CS antibody (17719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, HeLa cells, HepG2 cells, MCF-7 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

17719-1-AP targets P5CS in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P5CS fusion protein Ag12100
• Full Name: aldehyde dehydrogenase 18 family, member A1
• Calculated Molecular Weight: 795 aa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC106054
• Gene Symbol: P5CS
• Gene ID (NCBI): 5832
• RRID: AB_2223896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Sci Adv; Loss of attachment promotes proline accumulation and excretion in cancer cells; Authors - Steven E Pilley
human	WB	J Exp Med; Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.; Authors - Miao Liu ; KD Validated
mouse	WB	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai
human	WB	Proc Natl Acad Sci U S A; Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.; Authors - Liu Wei W
mouse	WB	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key
human	WB	Sci Rep; Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.; Authors - Wei Liu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kristian (Verified Customer) (02-05-2020)

The cells were collected, then lyzed five minutes at 95C in SDS-loading buffer.The lysate was run on a 4-20% denaturing SDS-PAGE, then blotted unto a nitrocellulose membrane. The protein was detected using primary antibody diluted 1:1,000 in blotto (PBS, 5% milk, 0.1% Tween-20), then secondary in a 1:10,000 dilution in blotto.Detected using ECL reagents.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human kidney cancer cells (RCC4)

P5CS Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:17719-1-AP)