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  • KD/KO Validated

P5CS Polyclonal antibody

P5CS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17719-1-AP

Synonyms

ALDH18A1, GK, GPR, GSAS, P5CS, PYCS



Tested Applications

Positive WB detected inHEK-293T cells, mouse brain tissue, HeLa cells, HepG2 cells, MCF-7 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17719-1-AP targets P5CS in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P5CS fusion protein Ag12100
Full Name aldehyde dehydrogenase 18 family, member A1
Calculated molecular weight 795 aa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC106054
Gene symbol ALDH18A1
Gene ID (NCBI) 5832
RRIDAB_2223896
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for P5CS antibody 17719-1-APDownload protocol
IHC protocol for P5CS antibody 17719-1-APDownload protocol
IF protocol for P5CS antibody 17719-1-APDownload protocol
IP protocol for P5CS antibody 17719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Loss of attachment promotes proline accumulation and excretion in cancer cells

Authors - Steven E Pilley
humanWB

J Exp Med

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Authors - Miao Liu
  • KD Validated
mouseWB

Autophagy

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.

Authors - Jingru Bai
humanWB

Proc Natl Acad Sci U S A

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Authors - Liu Wei W
mouseWB

Int J Mol Sci

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling

Authors - Jana Key
humanWB

Sci Rep

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Authors - Wei Liu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kristian (Verified Customer) (02-05-2020)

The cells were collected, then lyzed five minutes at 95C in SDS-loading buffer.The lysate was run on a 4-20% denaturing SDS-PAGE, then blotted unto a nitrocellulose membrane. The protein was detected using primary antibody diluted 1:1,000 in blotto (PBS, 5% milk, 0.1% Tween-20), then secondary in a 1:10,000 dilution in blotto.Detected using ECL reagents.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human kidney cancer cells (RCC4)
P5CS Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:17719-1-AP)