Validation Data Gallery
|Positive WB detected in
|HEK-293T cells, mouse brain tissue, HeLa cells, HepG2 cells, MCF-7 cells, rat brain tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17719-1-AP targets P5CS in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|P5CS fusion protein Ag12100
|aldehyde dehydrogenase 18 family, member A1
|Calculated molecular weight
|795 aa, 87 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.
|Product Specific Protocols
|WB protocol for P5CS antibody 17719-1-AP
|IHC protocol for P5CS antibody 17719-1-AP
|IF protocol for P5CS antibody 17719-1-AP
|IP protocol for P5CS antibody 17719-1-AP
|Click here to view our Standard Protocols
Loss of attachment promotes proline accumulation and excretion in cancer cells
J Exp Med
Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.
Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.
Proc Natl Acad Sci U S A
Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.
Int J Mol Sci
Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling
Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kristian (Verified Customer) (02-05-2020)
The cells were collected, then lyzed five minutes at 95C in SDS-loading buffer.The lysate was run on a 4-20% denaturing SDS-PAGE, then blotted unto a nitrocellulose membrane. The protein was detected using primary antibody diluted 1:1,000 in blotto (PBS, 5% milk, 0.1% Tween-20), then secondary in a 1:10,000 dilution in blotto.Detected using ECL reagents.