P5CS Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 87037-1-RR (same clone as 87037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87037-1-RR (P5CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

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  WB analysis using 87037-1-RR (same clone as 87037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87037-1-RR (P5CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87037-1-RR (same clone as 87037-1-PBS)

  Mouse colon tissue were subjected to SDS PAGE followed by western blot with 87037-1-RR (P5CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02872-1

  The mean P5CS concentration was determined to be 749.95 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 1,046.66 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02872-1

  Sandwich ELISA standard curve of MP02872-1, Human GNAS Recombinant Matched Antibody Pair - PBS only. 87037-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12100. 87037-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 ng/mL

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  IHC staining of rat brain using 87037-1-RR (same clone as 87037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 87037-1-RR (P5CS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 87037-1-RR (same clone as 87037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 87037-1-RR (P5CS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 87037-1-RR (same clone as 87037-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P5CS antibody (87037-1-RR, Clone: 252125B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

P5CS Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Cat No. 87037-1-PBS

Clone No.252125B12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

ALDH18A1, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase, Gamma-glutamyl phosphate reductase

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Product Information

87037-1-PBS targets P5CS as part of a matched antibody pair:

MP02872-1: 87037-2-PBS capture and 87037-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12100

  Product name: Recombinant human ALDH18A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-362 aa of BC106054

  Sequence: MLSQVYRCGFQPFNQHLLPWVKCTTVFRSHCIQPSVIRHVRSWSNIPFITVPLSRTHGKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGIKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPT

• Full Name: aldehyde dehydrogenase 18 family, member A1
• Calculated Molecular Weight: 795 aa, 87 kDa
• Observed Molecular Weight: 75-87 kDa
• GenBank Accession Number: BC106054
• Gene Symbol: P5CS
• Gene ID (NCBI): 5832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54886
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS and PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.