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ALDH1A1-specific Polyclonal antibody
ALDH1A1-specific Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 22109-1-AP
|Positive WB detected in||human liver tissue, mouse liver tissue, human adrenal gland tissue, K-562 cells|
|Positive IHC detected in||human liver tissue, human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22109-1-AP is ALDH1A1-specific Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ALDH1A1-specific fusion protein Ag17358|
|Full Name||aldehyde dehydrogenase 1 family, member A1|
|Calculated molecular weight||501 aa, 55 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC001505|
|Gene ID (NCBI)||216|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family. This antibody is specific to ALDH1A1.
|Product Specific Protocols|
|WB protocol for ALDH1A1-specific antibody 22109-1-AP||Download protocol|
|IHC protocol for ALDH1A1-specific antibody 22109-1-AP||Download protocol|
|IF protocol for ALDH1A1-specific antibody 22109-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Renal cell carcinoma with synchronous ipsilateral urothelial carcinoma of the renal pelvis.
PRMT3 interacts with ALDH1A1 and regulates gene-expression by inhibiting retinoic acid signaling.