ALDH1A2 Polyclonal antibody

ALDH1A2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 13951-1-AP

Synonyms

ALDH1A2, RALDH 2, RALDH(II), RALDH2, RALDH2 T, Retinal dehydrogenase 2

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Validation Data Gallery

• 

  WB analysis of K-562 using 13951-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13951-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13951-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13951-1-AP

  IP result of anti-ALDH1A2 (IP:13951-1-AP, 4ug; Detection:13951-1-AP 1:300) with mouse testis tissue lysate 4400ug.

• 

  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 5 publications below

Product Information

13951-1-AP targets ALDH1A2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH1A2 fusion protein Ag5062
• Full Name: aldehyde dehydrogenase 1 family, member A2
• Calculated Molecular Weight: 518 aa, 57 kDa
• Observed Molecular Weight: 53-57 kDa
• GenBank Accession Number: BC030589
• Gene Symbol: ALDH1A2
• Gene ID (NCBI): 8854
• RRID: AB_2224033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
human	WB	iScience; Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression; Authors - Zhilei Zhang
human	WB	Am J Cancer Res; Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression; Authors - Bing-Yen Wang
human	IHC	J Lipid Res; Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations.; Authors - Samuel L Arnold
mouse	WB	PLoS One; Retinoic acid signalling is activated in the postischemic heart and may influence remodelling.; Authors - Bilbija Dusan D