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ALDOA Polyclonal antibody

ALDOA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11217-1-AP

Synonyms

ALDOA, Aldolase A



Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11217-1-AP targets ALDOA in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDOA fusion protein Ag1726
Full Name aldolase A, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC016800
Gene symbol ALDOA
Gene ID (NCBI) 226
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Protocols

Product Specific Protocols
WB protocol for ALDOA antibody 11217-1-APDownload protocol
IHC protocol for ALDOA antibody 11217-1-APDownload protocol
IF protocol for ALDOA antibody 11217-1-APDownload protocol
IP protocol for ALDOA antibody 11217-1-APDownload protocol
FC protocol for ALDOA antibody 11217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
mouseWB

Nat Neurosci

Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.

Authors - Xiaoxiang Dong
mouseWB

Dev Cell

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.

Authors - Vinay Bulusu
humanIF

Mol Ther

Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.

Authors - Junyi Hu
mouseWB

eLife

Glycolytic flux-signaling controls mouse embryo mesoderm development

Authors - Hidenobu Miyazawa
humanCoIP

Oncogene

Upregulation of the ALDOA/DNA-PK/p53 pathway by dietary restriction suppresses tumor growth.

Authors - D Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

K (Verified Customer) (12-20-2020)

Worked well for IHC at 1:200 dilution

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse liver tissues and rat liver