ALG14 Polyclonal antibody

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  IHC staining of mouse kidney using 16207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16207-1-AP (ALG14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16207-1-AP (ALG14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16207-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ALG14 antibody (16207-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ALG14 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 16207-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Asparagine-linked glycosylation 14 homolog, UDP-N-acetylglucosamine transferase subunit ALG14

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Tested Applications

• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16207-1-AP targets ALG14 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9178

  Product name: Recombinant human ALG14 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-216 aa of BC011706

  Sequence: RSMDVTPRESLSILVVAGSGGHTTEILRLLGSLSNAYSPRHYVIADTDEMSANKINSFELDRADRDPSNMYTKYYIHRIPRSREVQQSWPSTVFTTLHSMWLSFPLIHRVKPDLVLCNGPGTCVPICVSALLLGILGIKKVIIVYVESICRVETLSMSGKILFHLSDYFIVQWPALKEKYPKSVYLGRIV

• Full Name: asparagine-linked glycosylation 14 homolog (S. cerevisiae)
• Calculated Molecular Weight: 216 aa, 24 kDa
• GenBank Accession Number: BC011706
• Gene Symbol: ALG14
• Gene ID (NCBI): 199857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96F25
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALG14 (UDP-N-Acetylglucosaminyltransferase Subunit ALG14 Homolog) is a protein involved in the process of N-linked glycosylation, which is essential for protein folding and stability. ALG14 forms a heterodimeric UDP-N-acetylglucosamine transferase (GnTase) with ALG13, which catalyzes a key step in the synthesis of the lipid-linked oligosaccharide (LLO) precursor during N-linked glycosylation (PMID: 36200043).