ALPL Polyclonal antibody

ALPL Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 11187-1-AP


Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, ALPL, BLAP, Bone Alkaline Phosphatase, NBAP

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11187-1-AP targets ALPL in WB, IF, FC, IHC, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALPL fusion protein Ag1664
Full Name alkaline phosphatase, liver/bone/kidney
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC021289
Gene Symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.


Product Specific Protocols
WB protocol for ALPL antibody 11187-1-APDownload protocol
IHC protocol for ALPL antibody 11187-1-APDownload protocol
IF protocol for ALPL antibody 11187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


CHAO (Verified Customer) (03-31-2022)

Weak bands may due to tissue specificity

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart

LUNFENG (Verified Customer) (01-27-2020)


  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART

Jie (Verified Customer) (01-23-2020)

Smear band but with the right product size on western blot. Immunohistochemical staining showed desired results in control versus knockout mouse model

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse colon tissue

Jingwen (Verified Customer) (01-17-2020)

This antibody worked very well and showed the specific band around 70kDa in western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart section