AMDHD1 Polyclonal antibody

AMDHD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21171-1-AP

Synonyms

AMDHD1, HMFT1272, Probable imidazolonepropionase

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 21171-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21171-1-AP (AMDHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 21171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21171-1-AP (AMDHD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21171-1-AP (AMDHD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21171-1-AP targets AMDHD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMDHD1 fusion protein Ag15420
• Full Name: amidohydrolase domain containing 1
• Calculated Molecular Weight: 426 aa, 47 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC029146
• Gene Symbol: AMDHD1
• Gene ID (NCBI): 144193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMDHD1 is a protein-coding gene that encodes the enzyme imidazolonepropionase. It is involved in the histidine catabolic process and has one potential isoform. The protein of AMDHD1 is located in the cytoplasm, and is expressed in all tissues, mainly in the liver, kidney and adrenal gland.