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CoraLite®488-conjugated AMPK Alpha Monoclonal antibody
AMPK Alpha Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse
Applications
IF
Conjugate
CoraLite®488 Fluorescent Dye
CloneNo.
1H8E1
Cat no : CL488-66536
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | human breast cancer tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66536 targets AMPK Alpha in IF applications and shows reactivity with Human, Rat, Mouse samples.
Tested Reactivity | Human, Rat, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AMPK Alpha fusion protein Ag24744 |
Full Name | protein kinase, AMP-activated, alpha 1 catalytic subunit |
Calculated molecular weight | 63 kDa |
Observed molecular weight | 64 kDa |
GenBank accession number | BC012622 |
Gene symbol | PRKAA1 |
Gene ID (NCBI) | 5562 |
Conjugate | CoraLite®488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
Protocols
Product Specific Protocols | |
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IF protocol for CL488 AMPK Alpha antibody CL488-66536 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |