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CoraLite®488-conjugated AMPK Alpha Monoclonal antibody

AMPK Alpha Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1H8E1

Cat no : CL488-66536

Synonyms

AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1



Tested Applications

Positive IF detected inhuman breast cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 66536-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66536 targets AMPK Alpha in IF applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMPK Alpha fusion protein Ag24744
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Calculated molecular weight 63 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC012622
Gene symbol PRKAA1
Gene ID (NCBI) 5562
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL488 AMPK Alpha antibody CL488-66536Download protocol
Standard Protocols
Click here to view our Standard Protocols