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- KD/KO Validated
AMPK Alpha Monoclonal antibody
AMPK Alpha Monoclonal Antibody for IHC, WB,ELISA
Cat no : 66536-1-Ig
|Positive WB detected in||HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, A549 cells, HepG2 cells, K-562 cells, PC-12 cells, L02 cells, HT-29 cells, THP-1 cells, 4T1 cells, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66536-1-Ig targets AMPK Alpha in WB, IHC,ELISA applications and shows reactivity with Human, Rat, Mouse samples.
|Tested Reactivity||Human, Rat, Mouse|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||AMPK Alpha fusion protein Ag24744|
|Full Name||protein kinase, AMP-activated, alpha 1 catalytic subunit|
|Calculated molecular weight||63 kDa|
|Observed molecular weight||64 kDa|
|GenBank accession number||BC012622|
|Gene ID (NCBI)||5562|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
Melatonin inhibits oxalate-induced endoplasmic reticulum stress and apoptosis in HK-2 cells by activating the AMPK pathway.
Stem Cells Int
Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway.
Cell Death Dis
WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.
Exp Ther Med
Paeoniflorin suppresses allergic and inflammatory responses by promoting autophagy in rats with urticaria.
Int J Environ Res Public Health
Impacts of Aerobic Exercise on Depression-Like Behaviors in Chronic Unpredictable Mild Stress Mice and Related Factors in the AMPK/PGC-1α Pathway.
J Physiol Biochem
Hesperetin inhibits foam cell formation and promotes cholesterol efflux in THP-1-derived macrophages by activating LXRα signal in an AMPK-dependent manner.