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AMPK Alpha Monoclonal antibody

AMPK Alpha Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (3)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1H8E1

Cat no : 66536-1-Ig

Synonyms

AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, A549 cells, HepG2 cells, K-562 cells, PC-12 cells, L02 cells, HT-29 cells, THP-1 cells, 4T1 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66536-1-Ig targets AMPK Alpha in WB, IHC,ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMPK Alpha fusion protein Ag24744
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Calculated molecular weight 63 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC012622
Gene symbol PRKAA1
Gene ID (NCBI) 5562
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AMPK Alpha antibody 66536-1-IgDownload protocol
IHC protocol for AMPK Alpha antibody 66536-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

Melatonin inhibits oxalate-induced endoplasmic reticulum stress and apoptosis in HK-2 cells by activating the AMPK pathway.

Authors - Qianlin Song
humanWB

Stem Cells Int

Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway.

Authors - Shuo Zhang
humanWB

Cell Death Dis

WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.

Authors - Zhe Zheng
ratIHC

Exp Ther Med

Paeoniflorin suppresses allergic and inflammatory responses by promoting autophagy in rats with urticaria.

Authors - Jing Guo
mouseWB

Int J Environ Res Public Health

Impacts of Aerobic Exercise on Depression-Like Behaviors in Chronic Unpredictable Mild Stress Mice and Related Factors in the AMPK/PGC-1α Pathway.

Authors - Jia Luo
mouseWB

Front Physiol

QiDiTangShen Granules Activate Renal Nutrient-Sensing Associated Autophagy in db/db Mice.

Authors - Xiangming Wang