ANGPTL3 Polyclonal antibody

ANGPTL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11964-1-AP

Synonyms

ANG 5, Angiopoietin 5, angiopoietin like 3, Angiopoietin like protein 3, Angiopoietin related protein 3, ANGPT5, ANGPTL3

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Validation Data Gallery

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  WB analysis of A375 using 11964-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11964-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 11964-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 11964-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A375 cells, mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11964-1-AP targets ANGPTL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANGPTL3 fusion protein Ag2573
• Full Name: angiopoietin-like 3
• Calculated Molecular Weight: 460 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC058287
• Gene Symbol: ANGPTL3
• Gene ID (NCBI): 27329
• RRID: AB_874076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.

Publications

Species	Application	Title
human,rat	WB	Invest Ophthalmol Vis Sci; PMID: 30128492; Authors - Na Wang
human	WB	Cancer Sci; Screening of novel serum biomarkers for gastric cancer in coastal populations using a protein microarray; Authors - Yongdong Yi