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ANGPTL4 Polyclonal antibody

ANGPTL4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18374-1-AP

Synonyms

angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL4, ARP4, FIAF, HFARP, NL2, PGAR, pp1158, PSEC0166, UNQ171/PRO197



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IF detected inNIH/3T3 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18374-1-AP is ANGPTL4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANGPTL4 fusion protein Ag13237
Full Name angiopoietin-like 4
Calculated molecular weight 45 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC023647
Gene symbol ANGPTL4
Gene ID (NCBI) 51129
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Protocols

Product Specific Protocols
WB protocol for ANGPTL4 antibody 18374-1-APDownload protocol
IHC protocol for ANGPTL4 antibody 18374-1-APDownload protocol
IF protocol for ANGPTL4 antibody 18374-1-APDownload protocol
IP protocol for ANGPTL4 antibody 18374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

J Clin Invest

Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.

Authors - Akrit Sodhi
mouseWB

EMBO J

LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Authors - Yanying Wang
mouseIF

J Pathol

ANGPTL4-αvβ3 integrin interaction counteracts hypoxia-induced vascular permeability by modulating Src signalling downstream of VEGFR2.

Authors - Elisa Gomez Perdiguero
humanIHC

Breast Cancer Res

An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-beta in human breast cancer.

Authors - Misako Sato
humanIHC,WB

J Cell Mol Med

Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure.

Authors - Mingyang Li
humanWB,IHC

J Cell Mol Med

The long non-coding RNA PVT1 represses ANGPTL4 transcription through binding with EZH2 in trophoblast cell.

Authors - Yetao Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Niklas (Verified Customer) (11-28-2022)

bright and specific staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:350
  • Cell Tissue Type: human fibroblasts