ANGPTL4 Monoclonal antibody

ANGPTL4 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D2H6

Cat no : 67577-1-Ig

Synonyms

angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL4, ARP4, FIAF, HFARP, NL2, PGAR, pp1158, PSEC0166, UNQ171/PRO197

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Validation Data Gallery

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  WB analysis of human placenta using 67577-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67577-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67577-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67577-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 67577-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67577-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67577-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 67577-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (67577-1-Ig, Clone: 1D2H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, COLO 320 cells, Caco-2 cells, HT-29 cells, mouse brain tissue, A549 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

67577-1-Ig targets ANGPTL4 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANGPTL4 fusion protein Ag30070
• Full Name: angiopoietin-like 4
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC023647
• Gene Symbol: ANGPTL4
• Gene ID (NCBI): 51129
• RRID: AB_2882790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Publications

Species	Application	Title
human	WB,IF	BMC Biol; Molecular, cellular and neurological consequences of infection by the neglected human pathogen Nocardia; Authors - Xingzhao Ji