ANO3 Polyclonal antibody

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• ANO3 Polyclonal antibody (19489-1-AP)

ANO3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 19489-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IP, ELISA

Synonyms

Transmembrane protein 16C, TMEM16C, GENX-3947, C11orf25, Anoctamin-3

• 

  WB analysis using 19489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 19489-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skin using 19489-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 19489-1-AP

  IP result of anti-ANO3 (IP:19489-1-AP, 4ug; Detection:19489-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skin tissue, mouse ovary tissue, mouse kidney tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19489-1-AP targets ANO3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: anoctamin 3
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 100-115 kDa
• GenBank Accession Number: NM_031418
• Gene Symbol: ANO3
• Gene ID (NCBI): 63982
• RRID: AB_10638489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BYT9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANO3, also named as C11orf25, TMEM16C and GENX-3947, belongs to the anoctamin family. It may act as a calcium-activated chloride channel.

Publications

What published studies show

The ANO3 Polyclonal antibody (Cat# 19489-1-AP) has 2 citations. One appears in Cell (2025), studying intrinsic heterogeneity of primary cilia through spatial proteomics in human samples using IF. The other is in Neuroscience Bulletin (2021), investigating the role of TMEM16C/Slack impairment in excitatory nociceptive synaptic plasticity and remifentanil-induced hyperalgesia in rats, using IP and IF. The research fields span cilia biology/spatial proteomics and pain neuroscience/synaptic plasticity.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell; Intrinsic heterogeneity of primary cilia revealed through spatial proteomics.; Authors - Jan N Hansen
rat	IP, IF	Neurosci Bull; A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats.; Authors - Yize Li