ANO3 Polyclonal antibody

ANO3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19489-1-AP

Synonyms

ANO3, anoctamin 3, C11orf25, GENX 3947, TMEM16C, Transmembrane protein 16C

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Validation Data Gallery

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  WB analysis using 19489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 19489-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19489-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19489-1-AP

  IP result of anti-ANO3 (IP:19489-1-AP, 4ug; Detection:19489-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skin tissue, mouse ovary tissue, mouse kidney tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• IP: See 1 publications below

Product Information

19489-1-AP targets ANO3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: anoctamin 3
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 100-115 kDa
• GenBank Accession Number: NM_031418
• Gene Symbol: ANO3
• Gene ID (NCBI): 63982
• RRID: AB_10638489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANO3, also named as C11orf25, TMEM16C and GENX-3947, belongs to the anoctamin family. It may act as a calcium-activated chloride channel.

Publications

Species	Application	Title
rat	IP, IF	Neurosci Bull; A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats.; Authors - Yize Li