JUN Polyclonal antibody

JUN Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22114-1-AP

Synonyms

Activator protein 1, AP 1, AP1, AP1,JUN,P39, c Jun, JUN, jun oncogene, P39, Proto oncogene c Jun, Transcription factor AP 1

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 22114-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22114-1-AP (JUN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22114-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22114-1-AP (JUN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22114-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22114-1-AP (JUN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 22114-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22114-1-AP (JUN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, rat brain tissue, HEK-293 cells
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below

Product Information

The immunogen of 22114-1-AP is JUN Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JUN fusion protein Ag17419
• Full Name: jun oncogene
• Calculated molecular weight: 331 aa, 36 kDa
• Observed molecular weight: 40-46 kDa
• GenBank accession number: BC002646
• Gene symbol: JUN
• Gene ID (NCBI): 3725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Front Physiol; Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.; Authors - Raul Chavez-Valdez
rat	WB	Exp Ther Med; Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation.; Authors - Ruichen Wang
mouse	WB	Molecules; Effects of the total saponins from Rosa laevigata Michx fruit against acetaminophen-induced liver damage in mice via induction of autophagy and suppression of inflammation and apoptosis.; Authors - Deshi Dong
human	WB	J Neuroinflammation; Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.; Authors - Ceshu Gao