AP2B1 Monoclonal antibody

AP2B1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a


Human, Mouse, Rat, Rabbit, Pig







Cat no : 68349-1-Ig


ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1, DKFZp781K0743

Tested Applications

Positive WB detected inpig cerebellum tissue, HeLa cells, rabbit cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

68349-1-Ig targets AP2B1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AP2B1 fusion protein Ag8264
Full Name adaptor-related protein complex 2, beta 1 subunit
Calculated molecular weight 105 kDa
Observed molecular weight100-115 kDa
GenBank accession numberBC006201
Gene symbol AP2B1
Gene ID (NCBI) 163
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.


Product Specific Protocols
WB protocol for AP2B1 antibody 68349-1-IgDownload protocol
IF protocol for AP2B1 antibody 68349-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols