AP3D1 Polyclonal antibody

AP3D1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16454-1-AP

Synonyms

ADTD, AP 3 complex subunit delta, AP 3 complex subunit delta 1, AP3D1, Delta adaptin, hBLVR



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

16454-1-AP targets AP3D1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3D1 fusion protein Ag9390
Full Name adaptor-related protein complex 3, delta 1 subunit
Calculated molecular weight 1153 aa, 130 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC010065
Gene symbol AP3D1
Gene ID (NCBI) 8943
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Protocols

Product Specific Protocols
WB protocol for AP3D1 antibody 16454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

PLoS One

Adaptor protein-3: A key player in RBL-2H3 mast cell mediator release.

Authors - Elaine Zayas Marcelino da Silva