API5 Polyclonal antibody

API5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25689-1-AP

Synonyms

AAC 11, AAC11, Antiapoptosis clone 11 protein, API 5, API5, apoptosis inhibitor 5, FIF, Protein XAGL



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25689-1-AP is API5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen API5 fusion protein Ag22524
Full Name apoptosis inhibitor 5
Calculated molecular weight 58 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC017709
Gene symbol API5
Gene ID (NCBI) 8539
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human gene API5 encodes the protein Apoptosis inhibitor 5. This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of chemotherapeutic drugs. Diseases associated with API5 include colon adenocarcinoma, and cervical cancer.

Protocols

Product Specific Protocols
WB protocol for API5 antibody 25689-1-APDownload protocol
IHC protocol for API5 antibody 25689-1-APDownload protocol
IF protocol for API5 antibody 25689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Paeonol-mediated apoptosis of hepatocellular carcinoma cells by NF-κB pathway.

Authors - Qiang Li
WB

Gene

Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica.

Authors - Shuang Zhang
mouseIHC

Sci Rep

microRNA regulatory circuits in a mouse model of inherited retinal degeneration.

Authors - Arpad Palfi