APLP1 Polyclonal antibody

APLP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12305-2-AP

Synonyms

Amyloid like protein 1, APLP, APLP 1, APLP1



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

12305-2-AP targets APLP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APLP1 fusion protein Ag2951
Full Name amyloid beta (A4) precursor-like protein 1
Calculated molecular weight 650 aa, 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC012889
Gene symbol APLP1
Gene ID (NCBI) 333
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APLP1 antibody 12305-2-APDownload protocol
IHC protocol for APLP1 antibody 12305-2-APDownload protocol
IP protocol for APLP1 antibody 12305-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis.

Authors - Mahlon Angus Collins
mouseWB

Mol Cell Proteomics

Label-free quantitative proteomics of mouse cerebrospinal fluid detects BACE1 protease substrates in vivo.

Authors - Bastian Dislich
mouseWB

EMBO J

Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons.

Authors - Kuhn Peer-Hendrik PH