APLP1 Polyclonal antibody

APLP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12305-2-AP

Synonyms

Amyloid like protein 1, APLP, APLP 1, APLP1

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Validation Data Gallery

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  WB analysis of mouse brain using 12305-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12305-2-AP (APLP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12305-2-AP

  IP result of anti-APLP1 (IP:12305-2-AP, 4ug; Detection:12305-2-AP 1:600) with mouse brain tissue lysate 3160 ug.

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  IHC staining of human brain using 12305-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12305-2-AP (APLP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12305-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12305-2-AP (APLP1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

12305-2-AP targets APLP1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APLP1 fusion protein Ag2951
• Full Name: amyloid beta (A4) precursor-like protein 1
• Calculated Molecular Weight: 650 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC012889
• Gene Symbol: APLP1
• Gene ID (NCBI): 333
• RRID: AB_2056369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Sci Transl Med; Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans.; Authors - Sabrina Simoes
mouse	WB	EMBO J; Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons.; Authors - Kuhn Peer-Hendrik PH
mouse	WB	Mol Cell Proteomics; Label-free quantitative proteomics of mouse cerebrospinal fluid detects BACE1 protease substrates in vivo.; Authors - Bastian Dislich
human	WB	J Proteome Res; Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis.; Authors - Mahlon Angus Collins