APOB Polyclonal antibody

APOB Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20578-1-AP

Synonyms

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B, Apolipoprotein B 100, FLDB



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

20578-1-AP targets APOB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name apolipoprotein B (including Ag(x) antigen)
Calculated molecular weight 516 kDa
Observed molecular weight 130-150 kDa, 200-250 kDa, 400-520 kDa
GenBank accession numberNM_000384
Gene symbol APOB
Gene ID (NCBI) 338
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein B (ApoB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The ApoB gene encodes two different isoproteins through mRNA editing, ApoB-48 and ApoB-100. ApoB-100 is synthesized exclusively by the liver and is essential for the assembly of VLDL in the liver. ApoB-48 is synthesized exclusively by the small intestine and is essential for the assembly of chylomicrons in the intestine. ApoB-48 and ApoB-100 share a common N-terminal sequence, but ApoB-48 lacks ApoB-100's C-terminal LDL receptor binding region. It is well established that ApoB-100 levels are associated with coronary heart disease. This antibody recognizes both of ApoB-48 and ApoB-100.

Protocols

Product Specific Protocols
WB protocol for APOB antibody 20578-1-APDownload protocol
IHC protocol for APOB antibody 20578-1-APDownload protocol
IF protocol for APOB antibody 20578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

EBioMedicine

Macrophage-derived myeloid differentiation protein 2 plays an essential role in ox-LDL-induced inflammation and atherosclerosis.

Authors - Taiwei Chen
humanWB

Biosci Biotechnol Biochem

Effect and mechanism of ginsenoside Rg1-regulating hepatic steatosis in HepG2 cells induced by free fatty acid.

Authors - Yue Gao
humanWB

Gut Liver

Downregulation of GNAI3 Promotes the Pathogenesis of Methionine/Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease.

Authors - Hanzhang Zhu
humanWB

Sci Rep

The miR-378c-Samd1 circuit promotes phenotypic modulation of vascular smooth muscle cells and foam cells formation in atherosclerosis lesions.

Authors - Shengya Tian
mouseWB

J Biol Chem

Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.

Authors - Xiaoling Li
ratWB

J Biol Chem

Small sequence variations between two mammalian paralogs of the small GTPase SAR1 underlie functional differences in coat protein complex II assembly.

Authors - David B Melville