APOB Polyclonal antibody

APOB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 20578-1-AP


Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B, Apolipoprotein B 100, FLDB

Tested Applications

Positive WB detected inhuman plasma, HEK-293 cells, mouse liver tissue, human plasma tissue
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20578-1-AP targets APOB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name apolipoprotein B (including Ag(x) antigen)
Calculated Molecular Weight 516 kDa
Observed Molecular Weight150-250 kDa, 400-520 kDa
GenBank Accession NumberNM_000384
Gene Symbol APOB
Gene ID (NCBI) 338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).


Product Specific Protocols
WB protocol for APOB antibody 20578-1-APDownload protocol
IHC protocol for APOB antibody 20578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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