APOB Polyclonal antibody

APOB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 20578-1-AP

Synonyms

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B, Apolipoprotein B 100, FLDB

---

Validation Data Gallery

• 

  WB analysis using 20578-1-AP

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20578-1-AP

  mouse liver and human plasma were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20578-1-AP

  HEK-293 cells were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 20578-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 20578-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 20578-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 20578-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: human plasma, HEK-293 cells, mouse liver tissue, human plasma tissue
• Positive IHC detected in: human liver tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

20578-1-AP targets APOB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: apolipoprotein B (including Ag(x) antigen)
• Calculated Molecular Weight: 516 kDa
• Observed Molecular Weight: 150-250 kDa, 400-520 kDa
• GenBank Accession Number: NM_000384
• Gene Symbol: APOB
• Gene ID (NCBI): 338
• RRID: AB_10732938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

Publications

Species	Application	Title
human,mouse	WB	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
mouse	WB	Nat Metab; Liver RBFOX2 regulates cholesterol homeostasis via Scarb1 alternative splicing in mice; Authors - Helen A B Paterson
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu

Related Products

Apolipoprotein AI Monoclonal antibody

66206-1-Ig

LDLR Polyclonal antibody

10785-1-AP

Apolipoprotein A II Polyclonal antibody

16845-1-AP