Tested Applications
| Positive WB detected in | human plasma, HEK-293 cells, mouse liver tissue, human plasma tissue |
| Positive IHC detected in | human liver tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20578-1-AP targets APOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, rabbit, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | apolipoprotein B (including Ag(x) antigen) |
| Calculated Molecular Weight | 516 kDa |
| Observed Molecular Weight | 150-250 kDa, 400-520 kDa |
| GenBank Accession Number | NM_000384 |
| Gene Symbol | APOB |
| Gene ID (NCBI) | 338 |
| RRID | AB_10732938 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04114 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. Unlike humans and rabbits, rats and mice secrete both apoB-100- and apoB-48-containing lipoproteins from the liver as a result of the hepatic expression of APOBEC-1 (PMID: 9633945). This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for APOB antibody 20578-1-AP | Download protocol |
| IHC protocol for APOB antibody 20578-1-AP | Download protocol |
| WB protocol for APOB antibody 20578-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APOB Polyclonal antibody (Cat# 20578-1-AP) has 82 citations spanning high-impact journals including Nature, Science, Cell Metabolism, Nature Communications, Molecular Cell, EMBO Journal, and Journal of Clinical Investigation. Research fields covered include lipid metabolism, atherosclerosis, NAFLD/NASH, extracellular vesicle biology, obesity, lipoprotein assembly, and cancer. Cited applications include WB, IF, IHC, and ELISA across human, mouse, rat, rabbit, chicken, and hamster species.
| Species | Application | Title |
|---|---|---|
Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein. | ||
Cell Metab Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts. | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Cell Metab AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. | ||
Nat Metab Liver RBFOX2 regulates cholesterol homeostasis via Scarb1 alternative splicing in mice |
Reviews
What customers say
The antibody received a 5-star rating for its performance in Western Blot on HepG2 cells at a 1:4000 dilution. The reviewer reported strong, specific signal with no unspecific bands, and noted that the antibody bath could be reused up to four times, making it both effective and cost-efficient for repeated experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ann-Kathrin (Verified Customer) (10-06-2025) | Antibody works really well in Western Blot, no unspecific singals. Antibody bath is reusable up to 4 times
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