APOBEC4 Polyclonal antibody

APOBEC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17166-1-AP

Synonyms

Putative C->U-editing enzyme APOBEC-4, EC:3.5.4.-, C1orf169, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4

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Validation Data Gallery

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  WB analysis of HeLa using 17166-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17166-1-AP (APOBEC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17166-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17166-1-AP (APOBEC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 17166-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 17166-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human brain tissue, human heart tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17166-1-AP targets APOBEC4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOBEC4 fusion protein Ag10633
• Full Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 4 (putative)
• Calculated Molecular Weight: 367 aa, 42 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC021711
• Gene Symbol: APOBEC4
• Gene ID (NCBI): 403314
• RRID: AB_2057526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.