Tested Applications
| Positive WB detected in | human serum tissue, HepG2 cells, human plasma |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:800-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10520-1-AP targets APOD in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0812 Product name: Recombinant human APOD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 15-189 aa of BC007402 Sequence: FGAAEGQAFHLGKCPNPPVQENFDVNKYLGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYWILATDYENYALVYSCTCIIQLFHVDFAWILARNPNLPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLS Predict reactive species |
| Full Name | apolipoprotein D |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 21-33 kDa |
| GenBank Accession Number | BC007402 |
| Gene Symbol | APOD |
| Gene ID (NCBI) | 347 |
| RRID | AB_2057977 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05090 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for APOD antibody 10520-1-AP | Download protocol |
| IHC protocol for APOD antibody 10520-1-AP | Download protocol |
| WB protocol for APOD antibody 10520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Apolipoprotein D (APOD) antibody (Cat# 10520-1-AP) has 11 citations published in journals including Journal of Extracellular Vesicles, Science Advances, Frontiers in Immunology, Cell Molecular Life Sciences, Computational and Structural Biotechnology Journal, Cell Signaling, Clinical Proteomics, Neuropathology and Applied Neurobiology, and Biomarkers. Research fields span oncology (NSCLC, gastric cancer), cardiovascular disease, neurodegeneration, renal pathology, and placental biology.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Identification of a Biomarker Panel in Extracellular Vesicles Derived From Non-Small Cell Lung Cancer (NSCLC) Through Proteomic Analysis and Machine Learning | ||
Front Immunol Development and validation of an ARID1A-related immune genes risk model in evaluating prognosis and immune therapeutic efficacy for gastric cancer patients: a translational study | ||
Front Immunol A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma | ||
Cell Mol Life Sci Apolipoprotein D acts as a chaperone of Aβ peptide delaying its assembly into amyloid aggregates. | ||
Comput Struct Biotechnol J Uncovering the potential of APOD as a biomarker in gastric cancer: A retrospective and multi-center study |
