• Featured Product
  • KD/KO Validated

APOL1-Specific Monoclonal antibody

APOL1-Specific Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12D11

Cat no : 66124-1-Ig

Synonyms

APO L, APOL, APOL I, APOL1, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66124-1-Ig is APOL1-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen APOL1-Specific fusion protein Ag2016
Full Name apolipoprotein L, 1
Calculated molecular weight 44 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC017331
Gene symbol APOL1
Gene ID (NCBI) 8542
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.

Protocols

Product Specific Protocols
WB protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
IHC protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
IF protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
IP protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
FC protocol for APOL1-Specific antibody 66124-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

Sci Rep

Disruption of APOL1-miR193a Axis Induces Disorganization of Podocyte Actin Cytoskeleton.

Authors - Vinod Kumar
  • KD Validated
humanWB

Am J Physiol Cell Physiol

Disrupted APOL1-miR193a Axis Dedifferentiates Podocytes through Autophagy Blockade in APOL1 Risk Milieu.

Authors - Vinod Kumar
humanWB,IF

Biosci Rep

APOL1 Risk Variants Cause Podocytes Injury through Enhancing Endoplasmic Reticulum Stress.

Authors - Hongxiu Wen
humanWB,IF

Am J Pathol

Role of APOL1 in Human Parietal Epithelial Cell Transition.

Authors - Vinod Kumar
mouseIF

Dis Model Mech

Recessive, gain-of-function toxicity in an APOL1 BAC transgenic mouse model mirrors human APOL1 kidney disease.

Authors - Gizelle M McCarthy
humanWB

FEBS J

Alterations in Plasma Membrane Ion Channel Structures Stimulate NLRP3 Inflammasomes Activation in APOL1 Risk Milieu.

Authors - Alok Jha

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB.

  • Applications: Western Blot,