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CoraLite® Plus 647-conjugated APOL1-Specific Monoclonal antibody

APOL1-Specific Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1G12D11

Cat no : CL647-66124

Synonyms

APOL1, Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CoraLite® Plus 647-conjugated APOL1-Specific Monoc (CL647-66124)

    IF Staining of human liver using CL647-66124

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL647-66124 (APOL1-Specific antibody) at dilution of 1:50.

Tested Applications

Positive IF-P detected inhuman liver tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66124 targets APOL1-Specific in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen APOL1-Specific fusion protein Ag2016
Full Name apolipoprotein L, 1
Calculated Molecular Weight 44 kDa
GenBank Accession NumberBC017331
Gene Symbol APOL1
Gene ID (NCBI) 8542
RRIDAB_2883731
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody is conjugated with CL647, Ex/Em 650 nm/665 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 APOL1-Specific antibody CL647-66124Download protocol
Standard Protocols
Click here to view our Standard Protocols